Fiber-optic biosensor to assess circulating phagocyte activity by chemiluminescence

Moni Magrisso, Ohad Etzion, Greg Pilch, Alex Novodvoretz, Galit Perez-Avraham, Francisc Schlaeffer, Robert Marks

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

We describe herein the construction of a novel computerized multi-sample temperature-controlled luminometer for a fiber array-based biosensor to monitor circulating phagocyte activity. It can perform simultaneously integral measurements of chemiluminescence emitted from up to six samples containing less than 0.5 μl whole blood while the samples and detector do not change their position during the measurement cycle. The optical fibers in this luminometer are used as both light guides and solid phase sample holders. The latter feature of the instrument design simplifies the assessment process of both the extra-cellular and the intra-cellular parts of the phagocyte-emitted chemiluminescence using the same system. We describe some examples or proof of principle for the use of the biosensor. This new technology may find use in a wide range of analytical luminescence applications in biology, biophysics, biochemistry, toxicology and clinical medicine.

Original languageEnglish
Pages (from-to)1210-1218
Number of pages9
JournalBiosensors and Bioelectronics
Volume21
Issue number7
DOIs
StatePublished - 15 Jan 2006

Keywords

  • Biosensor
  • Blood
  • Chemiluminescence
  • Fiber-optics
  • Luminometer
  • Phagocyte

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