FITNESS, a CCT domain-containing protein, deregulates reactive oxygen species levels and leads to fine-tuning trade-offs between reproductive success and defence responses in Arabidopsis

Ana Virginia Osella, Diego Alberto Mengarelli, Julieta Mateos, Shuchao Dong, Marcelo J. Yanovsky, Salma Balazadeh, Estela Marta Valle, María Inés Zanor

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Environmental stresses are the major factors that limit productivity in plants. Here, we report on the function of an uncharacterized gene At1g07050, encoding a CCT domain-containing protein, from Arabidopsis thaliana. At1g07050 expression is highly repressed by oxidative stress. We used metabolomics, biochemical, and genomic approaches to analyse performance of transgenic lines with altered expression of At1g07050 under normal and oxidative stress conditions. At1g07050 overexpressing lines showed increased levels of reactive oxygen species (ROS), whereas knock-out mutants exhibited decreased levels of ROS and higher tolerance to oxidative stress generated in the chloroplast. Our results uncover a role for At1g07050 in cellular redox homeostasis controlling H2O2 levels, due to changes in enzymes, metabolites, and transcripts related to ROS detoxification. Therefore, we call this gene FITNESS. Additionally, several genes such as ACD6, PCC1, and ICS1 related to salicylic acid signalling and defence were found differentially expressed among the lines. Notably, FITNESS absence significantly improved seed yield suggesting an effective fine-tuning trade-off between reproductive success and defence responses.

Original languageEnglish
Pages (from-to)2328-2341
Number of pages14
JournalPlant, Cell and Environment
Volume41
Issue number10
DOIs
StatePublished - 1 Oct 2018
Externally publishedYes

Keywords

  • cell death
  • flowering time
  • metabolism
  • oxidative stress
  • photorespiration
  • seed yield

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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