FLU-LISA (fluorescence-linked immunosorbent assay): high-throughput antibody profiling using antigen microarrays

Shlomia Levy, Marwa Abd Alhadi, Asaf Azulay, Amit Kahana, Nir Bujanover, Roi Gazit, Maureen A. McGargill, Lilach M. Friedman, Tomer Hertz

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Vaccination and natural infection both elicit potent humoral responses that provide protection from subsequent infections. The immune history of an individual following such exposures is in part encoded by antibodies. While there are multiple immunoassays for measuring antibody responses, the majority of these methods measure responses to a single antigen. A commonly used method for measuring antibody responses is ELISA—a semiquantitative assay that is simple to perform in research and clinical settings. Here, we present FLU-LISA (fluorescence-linked immunosorbent assay)—a novel antigen microarray-based assay for rapid high-throughput antibody profiling. The assay can be used for profiling immunoglobulin (Ig) G, IgA and IgM responses to multiple antigens simultaneously, requiring minimal amounts of sample and antigens. Using several influenza and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen microarrays, we demonstrated the specificity and sensitivity of our novel assay and compared it with the traditional ELISA, using samples from mice, chickens and humans. We also showed that our assay can be readily used with dried blood spots, which can be collected from humans and wild birds. FLU-LISA can be readily used to profile hundreds of samples against dozens of antigens in a single day, and therefore offers an attractive alternative to the traditional ELISA.

Original languageEnglish
Pages (from-to)231-248
Number of pages18
JournalImmunology and Cell Biology
Volume101
Issue number3
DOIs
StateAccepted/In press - 25 Dec 2022

Keywords

  • Antibody profiling
  • antigen microarrays
  • ELISA
  • FLU-LISA
  • immune history

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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