Fluorescence-based kinetic analysis of miniaturized protein microarrays

Heman Kumar Ramiya Ramesh Babu, Levi A. Gheber

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Ideal monitoring devices should enjoy a combination of characteristics, e.g. high sensitivity, multiplexing, portability, short time-to-result (TTR). Typically, no device meets all of these demands since some of them are contradictory, to some extent. Herein, we present a miniaturized platform based on fluorescent detection, which is sensitive, readily allows multiplexing, and allows real-time monitoring of the signal, thus allowing extraction of kinetic information as well as drastic reduction of TTR. This is achieved via miniaturization of active spots, integration with microfluidics, and algorithmic approaches. We validate its performance by comparing with evanescent field excitation, which obtains similar results, however without the addition of the necessary complex hardware.

Original languageEnglish
Pages (from-to)290-299
Number of pages10
JournalBiosensors and Bioelectronics
Volume122
DOIs
StatePublished - 30 Dec 2018

Keywords

  • Fluorescence detection
  • Kinetic parameters
  • Microfluidic
  • Miniaturization
  • Multiplexed detection
  • Protein microarray

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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