Fluorescence coherence tomography

A. Bilenca, A. Ozcan, B. Bouma, G. Tearney

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

In this paper, we introduce a new form of cross-sectional, coherence-gated fluorescence imaging, which we term 'spectral-domain fluorescence coherence tomography' (SD-FCT). SD-FCT is accomplished by spectrally detecting self-interference of the spontaneous emission of fluorophores located along the axial (depth) dimension of the sample. We have built a first generation SD-FCT system that utilizes two opposing low numerical-aperture objective lenses in an interferometer and an imaging spectrometer for detecting self-interference of fluorescence emitted from a sample. Here, in proof-of-principle experiments we demonstrate cross-sectional profiling of layered fluorescence phantoms. Narrow (a few micrometers FWHM) axial point-spread functions, large ranging depths (a few hundreds of micrometers) and wide fields of view (>1 mm) were measured. Initial results suggest that SD-FCT may be a viable tool for the investigation of semi-transparent and selectively labeled fluorescent samples.

Original languageEnglish
Pages (from-to)7134-7143
Number of pages10
JournalOptics Express
Volume14
Issue number16
DOIs
StatePublished - 1 Jan 2006
Externally publishedYes

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