Fluorescence correlation spectroscopy analysis of segmental dynamics in actin filaments

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21 Scopus citations

Abstract

We adapt fluorescence correlation spectroscopy (FCS) formalism to the studies of the dynamics of semiflexible polymers and derive expressions relating FCS correlation function to the longitudinal and transverse mean-square displacements of polymer segments. The obtained relations do not depend on any specific model of polymer dynamics. We use the derived expressions to measure the dynamics of actin filaments in two experimental situations: filaments labeled at distinct positions and homogeneously labeled filaments. Both approaches give consistent results and allow to measure the temporal dependence of the segmental mean-square displacement over almost five decades in time, from ∼40 μs to μ2 s. These noninvasive measurements allow for a detailed quantitative comparison of the experimental data to the current theories of semiflexible polymer dynamics. Good quantitative agreement is found between the experimental results and theories explicitly accounting for the hydrodynamic interactions between polymer segments.

Original languageEnglish
Article number084903
JournalJournal of Chemical Physics
Volume125
Issue number8
DOIs
StatePublished - 8 Sep 2006

ASJC Scopus subject areas

  • Physics and Astronomy (all)
  • Physical and Theoretical Chemistry

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