Abstract
We present a fluorescence imaging method based on transport of intensity equation (TIE) in which focus fluorescence image is retrieved from defocused intensity images. The focus image can be estimated from the phase image retrieved by TIE and the use of inverse Fresnel propagation under the partially coherence of microbeads. We present results for the focus measurement from experimentally recorded defocus fluorescence images.
Original language | English |
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Article number | 1114001-56 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 11140 |
State | Published - 1 Jan 2019 |
Externally published | Yes |
Event | Biomedical Imaging and Sensing Conference 2019, BISC 2019 - Yokohama, Japan Duration: 22 Apr 2019 → 26 Apr 2019 |
Keywords
- Fluorescence imaging
- Fresnel propagation
- Phase imaging
- Phase retrieval
- Transport of intensity equation
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering