Fluorescence imaging of microbeads by transport of intensity equation

Sudheesh K. Rajput; Manoj Kumar; Xiangyu Quan; Osamu Matoba; Yasuhiro Awatsuji

Fluorescence imaging of microbeads by transport of intensity equation

Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Osamu Matoba, Yasuhiro Awatsuji

Research output: Contribution to journal › Conference article › peer-review

Abstract

We present a fluorescence imaging method based on transport of intensity equation (TIE) in which focus fluorescence image is retrieved from defocused intensity images. The focus image can be estimated from the phase image retrieved by TIE and the use of inverse Fresnel propagation under the partially coherence of microbeads. We present results for the focus measurement from experimentally recorded defocus fluorescence images.

Original language	English
Article number	1114001-56
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11140
State	Published - 1 Jan 2019
Externally published	Yes
Event	Biomedical Imaging and Sensing Conference 2019, BISC 2019 - Yokohama, Japan Duration: 22 Apr 2019 → 26 Apr 2019

Keywords

Fluorescence imaging
Fresnel propagation
Phase imaging
Phase retrieval
Transport of intensity equation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

@article{864eb926b9614ad7814d8be99119fe05,

title = "Fluorescence imaging of microbeads by transport of intensity equation",

abstract = "We present a fluorescence imaging method based on transport of intensity equation (TIE) in which focus fluorescence image is retrieved from defocused intensity images. The focus image can be estimated from the phase image retrieved by TIE and the use of inverse Fresnel propagation under the partially coherence of microbeads. We present results for the focus measurement from experimentally recorded defocus fluorescence images.",

keywords = "Fluorescence imaging, Fresnel propagation, Phase imaging, Phase retrieval, Transport of intensity equation",

author = "Rajput, {Sudheesh K.} and Manoj Kumar and Xiangyu Quan and Osamu Matoba and Yasuhiro Awatsuji",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Biomedical Imaging and Sensing Conference 2019, BISC 2019 ; Conference date: 22-04-2019 Through 26-04-2019",

year = "2019",

month = jan,

day = "1",

language = "English",

volume = "11140",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

}

TY - JOUR

T1 - Fluorescence imaging of microbeads by transport of intensity equation

AU - Rajput, Sudheesh K.

AU - Kumar, Manoj

AU - Quan, Xiangyu

AU - Matoba, Osamu

AU - Awatsuji, Yasuhiro

PY - 2019/1/1

Y1 - 2019/1/1

N2 - We present a fluorescence imaging method based on transport of intensity equation (TIE) in which focus fluorescence image is retrieved from defocused intensity images. The focus image can be estimated from the phase image retrieved by TIE and the use of inverse Fresnel propagation under the partially coherence of microbeads. We present results for the focus measurement from experimentally recorded defocus fluorescence images.

AB - We present a fluorescence imaging method based on transport of intensity equation (TIE) in which focus fluorescence image is retrieved from defocused intensity images. The focus image can be estimated from the phase image retrieved by TIE and the use of inverse Fresnel propagation under the partially coherence of microbeads. We present results for the focus measurement from experimentally recorded defocus fluorescence images.

KW - Fluorescence imaging

KW - Fresnel propagation

KW - Phase imaging

KW - Phase retrieval

KW - Transport of intensity equation

UR - http://www.scopus.com/inward/record.url?scp=85065725094&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:85065725094

SN - 0277-786X

VL - 11140

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 1114001-56

T2 - Biomedical Imaging and Sensing Conference 2019, BISC 2019

Y2 - 22 April 2019 through 26 April 2019

ER -

Fluorescence imaging of microbeads by transport of intensity equation

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this