Fluorescence imaging of microbeads by transport of intensity equation

Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Osamu Matoba, Yasuhiro Awatsuji

Research output: Contribution to journalConference articlepeer-review


We present a fluorescence imaging method based on transport of intensity equation (TIE) in which focus fluorescence image is retrieved from defocused intensity images. The focus image can be estimated from the phase image retrieved by TIE and the use of inverse Fresnel propagation under the partially coherence of microbeads. We present results for the focus measurement from experimentally recorded defocus fluorescence images.

Original languageEnglish
Article number1114001-56
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 1 Jan 2019
Externally publishedYes
EventBiomedical Imaging and Sensing Conference 2019, BISC 2019 - Yokohama, Japan
Duration: 22 Apr 201926 Apr 2019


  • Fluorescence imaging
  • Fresnel propagation
  • Phase imaging
  • Phase retrieval
  • Transport of intensity equation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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