Fluorescence polarization assay for endothelin-converting enzymes

S. Hazan; I. Fishov; M. Zamai; V. R. Caiolfa; A. H. Parola

doi:10.1016/0196-9781(95)00032-F

Fluorescence polarization assay for endothelin-converting enzymes

S. Hazan
, I. Fishov
, M. Zamai
, V. R. Caiolfa
, A. H. Parola

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The conversion of big endothelin to endothelin by α-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.

Original language	English
Pages (from-to)	833-836
Number of pages	4
Journal	Peptides
Volume	16
Issue number	5
DOIs	https://doi.org/10.1016/0196-9781(95)00032-F
State	Published - 1 Jan 1995

Keywords

Endothelin
Endothelin-converting enzyme assay
Fluorescence polarization
Tryptophan fluorescence

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/0196-9781(95)00032-F

Cite this

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title = "Fluorescence polarization assay for endothelin-converting enzymes",

abstract = "The conversion of big endothelin to endothelin by α-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.",

keywords = "Endothelin, Endothelin-converting enzyme assay, Fluorescence polarization, Tryptophan fluorescence",

author = "S. Hazan and I. Fishov and M. Zamai and Caiolfa, \{V. R.\} and Parola, \{A. H.\}",

year = "1995",

month = jan,

day = "1",

doi = "10.1016/0196-9781(95)00032-F",

language = "English",

volume = "16",

pages = "833--836",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "5",

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T1 - Fluorescence polarization assay for endothelin-converting enzymes

AU - Hazan, S.

AU - Fishov, I.

AU - Zamai, M.

AU - Caiolfa, V. R.

AU - Parola, A. H.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The conversion of big endothelin to endothelin by α-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.

AB - The conversion of big endothelin to endothelin by α-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.

KW - Endothelin

KW - Endothelin-converting enzyme assay

KW - Fluorescence polarization

KW - Tryptophan fluorescence

UR - https://www.scopus.com/pages/publications/0029120240

U2 - 10.1016/0196-9781(95)00032-F

DO - 10.1016/0196-9781(95)00032-F

M3 - Article

C2 - 7479323

AN - SCOPUS:0029120240

SN - 0196-9781

VL - 16

SP - 833

EP - 836

JO - Peptides

JF - Peptides

IS - 5

ER -

Fluorescence polarization assay for endothelin-converting enzymes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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