Abstract
Fourier-Transform Infrared Spectroscopy (FTIR) employs a unique approach to optical diagnosis of tissue pathology based on the characteristic molecular vibrational spectra of the tissue. The architectural changes in the cellular and sub-cellular levels developing in abnormal tissue, including a majority of cancer forms, manifest themselves in different optical signatures, which can be detected in infrared spectroscopy. The molecular vibrational modes, which are responsible for infrared (IR) absorption spectra, are characteristic of the biochemistry of the cells and their sub-cellular components. The biological systems we have studied include adenocarcinoma and normal colonic tissues obtained from the department of pathology at Soroka Medical Center (SMC). Our method is based on microscopic infrared study (FTIR-microscopy) of thin tissue specimens and a direct comparison with normal histopathological analysis, which serves as a `gold' reference. Several unique differences between normal and cancerous intestinal specimens have been observed. The cancerous intestine has weaker absorption strength over a wide region, which includes several significant vibrational bands. The results from microscopic IR absorption spectra from intestinal tissues (normal and cancerous) have also been compared with other biological tissue samples.
Original language | English |
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Pages (from-to) | 66-77 |
Number of pages | 12 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 3918 |
DOIs | |
State | Published - 1 Jan 2000 |
Event | Biomedical Spectroscopy: Vibrational Spectroscopy and other Novel Techniques - San Jose, CA, USA Duration: 26 Jan 2000 → 27 Jan 2000 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering