Fractionation and partial characterization of the sulphated polysaccharide of Porphyridium

S. Geresh, N. Lupescu, S. (Malis) Arad

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The carbohydrate composition of the extracellular sulphated polysaccharide obtained from cultures of the unicellular red alga Porphyridium was determined. The polysaccharide was separated by anion-exchange chromatography into eight fractions. The major carbohydrate-containing fractions, accounting for ca 89% of the total carbohydrates, were the 0.5, 1.0 and 2.0 M NaCl fractions and the hot urea fraction. The NaCl-eluted fractions showed very similar neutral sugar compositions, with some differences in the contents of sulphate and uronic acid, which increased from the 0.5 M to the 2 M NaCl fraction. The hot urea fraction was apparently a xylan (about 86% xylose) and accounted for about 72% of the protein found in the whole polysaccharide. These findings indicate the presence of at least two different polysaccharides, one of which seems to be a proteoglycan. Amino acid analysis of the latter fraction was performed.

Original languageEnglish
Pages (from-to)4181-4186
Number of pages6
JournalPhytochemistry
Volume31
Issue number12
DOIs
StatePublished - 1 Jan 1992

Keywords

  • Porphyridium sp., unicellular red algae
  • Rhodophyta
  • amino acid analysis.
  • anion-exchange chromatography
  • fractionation
  • gel-permeation chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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