Fresh and cryopreserved fetal bones replacing massive bone loss in rats

Z. Nevo, A. Lev-El, T. Siegal, C. Altaratz, S. Segal, S. Dolev, L. Nebel

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Cartilaginous fetal bones from rat preserved by deep freezing procedures were compared to comparable fresh bones with regard to the following parameters: chemical composition, water and uronic acid contents; cell viability measured by the rate of proteoglycan synthesis; mineralizationossification status by calcium binding; matrix integrity by the release of uronic acid containing substances; and biological activity as transplants inducing the formation of bone. The transplanted material was chemically analyzed and checked for its rate of proteoglycan synthesis. The quality of the formed bone was similar whether isogeneic or allogeneic, fresh or cryopreserved bone was employed as transplant material. Evidently those various fetal bones may be of clinical value whenever the need for replacement of massive bone loss arises. Although the viability and the cartilaginous nature of the graft are critical, the isogeneity and freshness are of a quantitative advantage only. These biochemical observations were confirmed by roentgenological and histological evaluations of the grafts. An optimal cryopreserving procedure and tests for examining bone candidates for successful grafting are described.

Original languageEnglish
Pages (from-to)62-69
Number of pages8
JournalCalcified Tissue International
Issue number1
StatePublished - 1 Dec 1983
Externally publishedYes


  • Bone banking
  • Bone transplants
  • Cartilage-Bone transformation
  • Chondrocyte viability
  • Connective tissue matrix integrity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology


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