FTIR microscopic studies on normal and H-ras oncogene transfected cultured mouse fibroblasts

David Bigman, Hippolyte Fofack

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Infrared absorption spectra are well known for their sensitivity to composition and three-dimensional structure of biomolecules. The biochemical changes in the sub-cellular levels developing in abnormal cells, including a majority of cancer forms, manifest themselves in different optical signatures, which can be detected by IR spectroscopy. We measured the IR absorption spectra of monolayers of cultured normal and H-ras transfected mouse fibroblasts, using a microscopic Fourier transform IR (micro-FTIR) technique. The absorption of normal cells was found to be higher than the malignant ones in the spectral range 600-3200 cm-1. The carbohydrate and phosphate contents were higher in normal cells relative to H-ras transfected cells. An increase in the RNA/DNA ratio was observed for H-ras transfected fibroblasts, which correlates with the increased transcriptional activity expected for the cancerous cells. In part, the variation in absorbance between normal and ras transfected fibroblasts may be due to changes in the cell dimensions.

Original languageEnglish
Pages (from-to)250-255
Number of pages6
JournalEuropean Biophysics Journal
Volume30
Issue number4
DOIs
StatePublished - 1 Jan 2001
Externally publishedYes

Keywords

  • Cancer
  • Fourier transform IR microscopy
  • Mouse fibroblasts
  • Ras oncogene
  • Spectral fitting

ASJC Scopus subject areas

  • Biophysics

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