TY - JOUR
T1 - FTIR microspectroscopy of malignant fibroblasts transformed by mouse sarcoma virus
AU - Salman, Ahmad
AU - Ramesh, Jagannathan
AU - Erukhimovitch, Vitaly
AU - Talyshinsky, Marina
AU - Mordechai, Shaul
AU - Huleihel, Mahmoud
N1 - Funding Information:
We gratefully acknowledge the Harry Stern Applied Research Grant Program and the Israel Science Foundation (ISF grant number: 788/01) for their financial support.
PY - 2003/2/28
Y1 - 2003/2/28
N2 - Fourier transform infrared microspectroscopy (FTIR-MSP), which is based on the characteristic molecular vibrational spectra of cells, was used to investigate spectral differences between normal primary rabbit bone marrow (BM) cells and bone marrow cells transformed (BMT) by murine sarcoma virus (MuSV). Primary cells, rather than cell lines, were used for this research because primary cells are similar to normal tissue cells in most of their characteristics. Our results showed dramatic changes in absorbance between the control cells and MuSV124-transformed cells. Various biological markers, such as the phosphate level and the RNA/DNA obtained, based on the analysis of the FTIR-MSP spectra, also displayed significant differences between the control and transformed cells. Preliminary results suggested that the cluster analysis performed on the FTIR-MSP spectra yielded 100% accuracy in classifying both types of cells.
AB - Fourier transform infrared microspectroscopy (FTIR-MSP), which is based on the characteristic molecular vibrational spectra of cells, was used to investigate spectral differences between normal primary rabbit bone marrow (BM) cells and bone marrow cells transformed (BMT) by murine sarcoma virus (MuSV). Primary cells, rather than cell lines, were used for this research because primary cells are similar to normal tissue cells in most of their characteristics. Our results showed dramatic changes in absorbance between the control cells and MuSV124-transformed cells. Various biological markers, such as the phosphate level and the RNA/DNA obtained, based on the analysis of the FTIR-MSP spectra, also displayed significant differences between the control and transformed cells. Preliminary results suggested that the cluster analysis performed on the FTIR-MSP spectra yielded 100% accuracy in classifying both types of cells.
KW - Cluster analysis
KW - FTIR microspectroscopy
KW - Fibroblast
KW - MuSV
UR - http://www.scopus.com/inward/record.url?scp=0037469544&partnerID=8YFLogxK
U2 - 10.1016/S0165-022X(02)00182-3
DO - 10.1016/S0165-022X(02)00182-3
M3 - Article
AN - SCOPUS:0037469544
SN - 0165-022X
VL - 55
SP - 141
EP - 153
JO - Journal of Biochemical and Biophysical Methods
JF - Journal of Biochemical and Biophysical Methods
IS - 2
ER -
