TY - JOUR
T1 - Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein
AU - Tabib-Salazar, Aline
AU - Liu, Bing
AU - Shadrin, Andrey
AU - Burchell, Lynn
AU - Wang, Zhexin
AU - Wang, Zhihao
AU - Goren, Moran G.
AU - Yosef, Ido
AU - Qimron, Udi
AU - Severinov, Konstantin
AU - Matthews, Steve J.
AU - Wigneshweraraj, Sivaramesh
N1 - Publisher Copyright:
© The Author(s) 2017.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the bacterial RNA polymerase (RNAP) by the 7 kDa T7 protein Gp2. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.7, which adopts a winged helix-turn-helix-like structure and specifically represses transcription initiation from host RNAP-dependent promoters on the phage genome via a mechanism that involves interaction with DNA and the bacterial RNAP. Whereas Gp2 is indispensable for T7 growth in E. coli, we show that Gp5.7 is required for optimal infection outcome. Our findings provide novel insights into how phages fine-tune the activity of the host transcription machinery to ensure both successful and efficient phage progeny development.
AB - Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the bacterial RNA polymerase (RNAP) by the 7 kDa T7 protein Gp2. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.7, which adopts a winged helix-turn-helix-like structure and specifically represses transcription initiation from host RNAP-dependent promoters on the phage genome via a mechanism that involves interaction with DNA and the bacterial RNAP. Whereas Gp2 is indispensable for T7 growth in E. coli, we show that Gp5.7 is required for optimal infection outcome. Our findings provide novel insights into how phages fine-tune the activity of the host transcription machinery to ensure both successful and efficient phage progeny development.
UR - http://www.scopus.com/inward/record.url?scp=85026383508&partnerID=8YFLogxK
U2 - 10.1093/nar/gkx370
DO - 10.1093/nar/gkx370
M3 - Article
C2 - 28486695
AN - SCOPUS:85026383508
SN - 0305-1048
VL - 45
SP - 7697
EP - 7707
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 13
ER -