Functional assay of plasminogen activator by hydrolysis of 14C-globin

Z. Finci; I. Nachshon; Y. Sharoni; M. Mayer

Functional assay of plasminogen activator by hydrolysis of ¹⁴C-globin

Z. Finci, I. Nachshon, Y. Sharoni, M. Mayer

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A functional method for estimation of plasminogen activator (PA) activity is described. The end-point method follows the PA-dependent conversion of the inactive plasminogen to the potent protease plasmin. The formation of plasmin is monitored by its activity on ¹⁴C-globin to form acid-soluble radioactive products. The globinolytic assay of PA shows sensitivity similar to that of the ¹²⁵I-fibrinolytic assay, and is superior in sensitivity to both the chromogenic (Pyro-Glu-Gly-Arg-pNA, S-2444) and the fluorimetric (Glu-Gly-Arg-methylcoumarin amide) methods. The assay is applicable for estimation of tissue type and urokinaselike PA activity in biologic samples, including viable cells in suspension and conditioned medium.

Original language	English
Pages (from-to)	293-297
Number of pages	5
Journal	Experimental Hematology
Volume	14
Issue number	4
State	Published - 1 Jan 1986
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Hematology
Genetics
Cell Biology
Cancer Research

Cite this

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title = "Functional assay of plasminogen activator by hydrolysis of 14C-globin",

abstract = "A functional method for estimation of plasminogen activator (PA) activity is described. The end-point method follows the PA-dependent conversion of the inactive plasminogen to the potent protease plasmin. The formation of plasmin is monitored by its activity on 14C-globin to form acid-soluble radioactive products. The globinolytic assay of PA shows sensitivity similar to that of the 125I-fibrinolytic assay, and is superior in sensitivity to both the chromogenic (Pyro-Glu-Gly-Arg-pNA, S-2444) and the fluorimetric (Glu-Gly-Arg-methylcoumarin amide) methods. The assay is applicable for estimation of tissue type and urokinaselike PA activity in biologic samples, including viable cells in suspension and conditioned medium.",

author = "Z. Finci and I. Nachshon and Y. Sharoni and M. Mayer",

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T1 - Functional assay of plasminogen activator by hydrolysis of 14C-globin

AU - Finci, Z.

AU - Nachshon, I.

AU - Sharoni, Y.

AU - Mayer, M.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - A functional method for estimation of plasminogen activator (PA) activity is described. The end-point method follows the PA-dependent conversion of the inactive plasminogen to the potent protease plasmin. The formation of plasmin is monitored by its activity on 14C-globin to form acid-soluble radioactive products. The globinolytic assay of PA shows sensitivity similar to that of the 125I-fibrinolytic assay, and is superior in sensitivity to both the chromogenic (Pyro-Glu-Gly-Arg-pNA, S-2444) and the fluorimetric (Glu-Gly-Arg-methylcoumarin amide) methods. The assay is applicable for estimation of tissue type and urokinaselike PA activity in biologic samples, including viable cells in suspension and conditioned medium.

AB - A functional method for estimation of plasminogen activator (PA) activity is described. The end-point method follows the PA-dependent conversion of the inactive plasminogen to the potent protease plasmin. The formation of plasmin is monitored by its activity on 14C-globin to form acid-soluble radioactive products. The globinolytic assay of PA shows sensitivity similar to that of the 125I-fibrinolytic assay, and is superior in sensitivity to both the chromogenic (Pyro-Glu-Gly-Arg-pNA, S-2444) and the fluorimetric (Glu-Gly-Arg-methylcoumarin amide) methods. The assay is applicable for estimation of tissue type and urokinaselike PA activity in biologic samples, including viable cells in suspension and conditioned medium.

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M3 - Article

AN - SCOPUS:0022550870

SN - 0301-472X

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SP - 293

EP - 297

JO - Experimental Hematology

JF - Experimental Hematology

IS - 4

ER -

Functional assay of plasminogen activator by hydrolysis of ¹⁴C-globin

Abstract

ASJC Scopus subject areas

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