Functional comparison of bone marrow-derived liver stem cells: Selection strategy for cell-based therapy

Daniel Inderbitzin, Itzhak Avital, Beat Gloor, Adrian Keogh, Daniel Candinas

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Several distinct subpopulations of bone marrow-derived liver progenitor cells were recently described. However, there is inadequate information comparing these subpopulations from a liver-function point of view. This study was undertaken to compare two subpopulations of liver progenitors: β2-microglobulin (β2m)-negative/Thy-1-positive cells, and liver progenitors obtained from the non-adherent cell fraction after a panning procedure. The cells were cultured under several conditions including high- and low-dose hepatocyte growth factor, various cellular densities, and different media. Growth characteristics, liver-specific metabolic capacity, and liver regeneration-associated gene expression were studied. Both isolation procedures yielded cells that produced albumin and metabolized ammonia into urea. The study demonstrated that the β2m-negative/Thy-1- positive cell fraction metabolized ammonia into urea more efficiently and produced a superior amount of albumin compared with the panned cell fraction. The β2m-negative/Thy-1-positive cell fraction could be optimal for the development of novel cell-based treatment strategies for congenital or acquired liver diseases.

Original languageEnglish
Pages (from-to)1340-1345
Number of pages6
JournalJournal of Gastrointestinal Surgery
Issue number9
StatePublished - 1 Dec 2005
Externally publishedYes


  • Cell biology
  • Hepatic stem cells
  • In vitro study
  • Metabolism
  • Rodent

ASJC Scopus subject areas

  • Surgery
  • Gastroenterology


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