Functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae

Nor Chejanovsky, Noam Zilberberg, Hadasah Rivkin, Eliahu Zlotkin, Michael Gurevitz

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The Leiurus quinquestriatus hebraeus alpha anti-insect toxin (LqhαIT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional LqhαIT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with wild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti-insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti-insect selectivity.

Original languageEnglish
Pages (from-to)181-184
Number of pages4
JournalFEBS Letters
Volume376
Issue number3
DOIs
StatePublished - 4 Dec 1995
Externally publishedYes

Keywords

  • Baculovirus
  • Insect cells
  • Lepidopterous larvae
  • LqhαIT (Leiurus quinquestriatus hebraeus) anti-insect toxin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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