Functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae

Nor Chejanovsky; Noam Zilberberg; Hadasah Rivkin; Eliahu Zlotkin; Michael Gurevitz

doi:10.1016/0014-5793(95)01263-4

Functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae

Nor Chejanovsky, Noam Zilberberg, Hadasah Rivkin, Eliahu Zlotkin, Michael Gurevitz

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The Leiurus quinquestriatus hebraeus alpha anti-insect toxin (LqhαIT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional LqhαIT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with wild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti-insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti-insect selectivity.

Original language	English
Pages (from-to)	181-184
Number of pages	4
Journal	FEBS Letters
Volume	376
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01263-4
State	Published - 4 Dec 1995
Externally published	Yes

Keywords

Baculovirus
Insect cells
Lepidopterous larvae
LqhαIT (Leiurus quinquestriatus hebraeus) anti-insect toxin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)01263-4

Cite this

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title = "Functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae",

abstract = "The Leiurus quinquestriatus hebraeus alpha anti-insect toxin (LqhαIT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional LqhαIT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with wild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti-insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti-insect selectivity.",

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author = "Nor Chejanovsky and Noam Zilberberg and Hadasah Rivkin and Eliahu Zlotkin and Michael Gurevitz",

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doi = "10.1016/0014-5793(95)01263-4",

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T1 - Functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae

AU - Chejanovsky, Nor

AU - Zilberberg, Noam

AU - Rivkin, Hadasah

AU - Zlotkin, Eliahu

AU - Gurevitz, Michael

PY - 1995/12/4

Y1 - 1995/12/4

N2 - The Leiurus quinquestriatus hebraeus alpha anti-insect toxin (LqhαIT) cDNA was engineered into the Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant virus secreted a functional LqhαIT polypeptide. Spodoptera littoralis and Heliothis armigera larvae injected with recombinant budded virus, showed typical intoxication symptoms. This recombinant virus showed enhanced insecticidal potency against H. armigera larvae compared with wild type AcNPV. The present expression system will facilitate: (1) the future elucidation of structural elements involved in its prominent anti-insect toxicity; and (2) the future design of genetically modified alpha toxins with improved anti-insect selectivity.

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KW - Baculovirus

KW - Insect cells

KW - Lepidopterous larvae

KW - LqhαIT (Leiurus quinquestriatus hebraeus) anti-insect toxin

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Functional expression of an alpha anti-insect scorpion neurotoxin in insect cells and lepidopterous larvae

Abstract

Keywords

ASJC Scopus subject areas

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