TY - JOUR
T1 - Functional modeling of electrochemical whole-cell biosensors
AU - Ben-Yoav, Hadar
AU - Biran, Alva
AU - Sternheim, Marek
AU - Belkin, Shimshon
AU - Freeman, Amihay
AU - Shacham-Diamand, Yosi
N1 - Funding Information:
This work was partially supported by the “Dip-chip” project funded by the German–Israeli BMBF-MOST Cooperation in Water Technology grant number WT0601/02WU0844 . The work was also supported by the Tel Aviv University Scholarship Fund . We wish to thank to Dr. Sefi Vernick for the multiple discussions on the model development. We also wish to thank Dr. Rami Pedahzur and Dr. Sharon Yagur-Kroll for the fruitful discussion and for the development and providing of the genetically engineered E. coli whole-cell biosensors. We are grateful to Dr. Sebastian Buchinger and Dr. Georg Reifferscheid for the fruitful discussion on toxicity assays.
PY - 2013/3/25
Y1 - 2013/3/25
N2 - The response modeling of whole-cell biochip represents the link between cellular biology and transducer output, allowing better system engineering. It provides the mathematical background for signal and noise modeling, performance prediction and data analysis. Here we describe an analytical model for whole-cell biosensors with electrochemical detection for single use, test and dispose applications. In this system the electrochemical signal is generated by the oxidation of the by-products of the reaction between an external substrate and the enzyme alkaline phosphatase. The enzyme expression can be either normal or enhanced due to the response of the biological cell to an external excitation. The electrochemical oxidation current is measured as a function of time. The model is based on the electrochemical reaction rate equations; an analytical solution is presented, compared to data and discussed.
AB - The response modeling of whole-cell biochip represents the link between cellular biology and transducer output, allowing better system engineering. It provides the mathematical background for signal and noise modeling, performance prediction and data analysis. Here we describe an analytical model for whole-cell biosensors with electrochemical detection for single use, test and dispose applications. In this system the electrochemical signal is generated by the oxidation of the by-products of the reaction between an external substrate and the enzyme alkaline phosphatase. The enzyme expression can be either normal or enhanced due to the response of the biological cell to an external excitation. The electrochemical oxidation current is measured as a function of time. The model is based on the electrochemical reaction rate equations; an analytical solution is presented, compared to data and discussed.
KW - Biochips
KW - Bioelectrochemistry
KW - Michaelis-Menten kinetics
KW - Modeling
KW - Whole-cell biosensors
UR - http://www.scopus.com/inward/record.url?scp=84875179279&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2013.02.032
DO - 10.1016/j.snb.2013.02.032
M3 - Article
AN - SCOPUS:84875179279
SN - 0925-4005
VL - 181
SP - 479
EP - 485
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -