TY - JOUR
T1 - Functionalized MoS2 nanosheets assembled microfluidic immunosensor for highly sensitive detection of food pathogen
AU - Singh, Chandan
AU - Ali, Md Azahar
AU - Kumar, Vinod
AU - Ahmad, Razi
AU - Sumana, Gajjala
N1 - Funding Information:
We thank Director NPL, New Delhi, India for the facilities. Chandan Singh is thankful to CSIR, India, for the award of a Senior Research Fellowship. Authors are thankful to Prof.(Dr.) Bansi D. Malhotra and Dr. R.K. Kotnala for encouragement and support. The authors are also thankful to Mr. Dinesh Singh and Mr. Jay Tawale for assistance in HRTEM and SEM studies, respectively.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Two dimensional nanomaterials such as molybdenum disulfide (MoS2) has gained significant interest in designing electrochemical devices for biosensing application. In this work, we have designed cetyltrimethyl ammonium bromide (CTAB) functionalized MoS2 nanosheets (CTAB-MoS2-NS) for conjugation of protein on the microfluidics electrode for Salmonella typhimurium (S.typhimurium) detection. MoS2 nanosheets from the bulk MoS2 were synthesized using CTAB assisted exfoliation methods resulting into positive charge on the CTAB-MoS2-NS. Further, optical and electron microscopic studies of CTAB-MoS2-NS reveal, its suitability for biosensing application. These CTAB-MoS2-NS were deposited onto the patterned hydrolyzed indium tin oxide (ITO) microelelctrode, utilizing the strong electrostatic interaction with the positively charged CTAB-MoS2-NS. Then microelectrode has been integrated with polydimethylsiloxane (PDMS) microfluidic device to design a Lab on a Chip platform for S.typhimurium detection. Detection of the S.typhimurium cells have been performed using electrochemical impedance spectroscopy (EIS) technique. The fabricated microfluidic immunosensor offers highly improved biosensing parameters, as sensitivity was found to be 1.79 kΩ/CFU−1mL cm−2 with the detection limit 1.56 CFU mL−1in the wide detection range of 101–107 CFUmL−1.
AB - Two dimensional nanomaterials such as molybdenum disulfide (MoS2) has gained significant interest in designing electrochemical devices for biosensing application. In this work, we have designed cetyltrimethyl ammonium bromide (CTAB) functionalized MoS2 nanosheets (CTAB-MoS2-NS) for conjugation of protein on the microfluidics electrode for Salmonella typhimurium (S.typhimurium) detection. MoS2 nanosheets from the bulk MoS2 were synthesized using CTAB assisted exfoliation methods resulting into positive charge on the CTAB-MoS2-NS. Further, optical and electron microscopic studies of CTAB-MoS2-NS reveal, its suitability for biosensing application. These CTAB-MoS2-NS were deposited onto the patterned hydrolyzed indium tin oxide (ITO) microelelctrode, utilizing the strong electrostatic interaction with the positively charged CTAB-MoS2-NS. Then microelectrode has been integrated with polydimethylsiloxane (PDMS) microfluidic device to design a Lab on a Chip platform for S.typhimurium detection. Detection of the S.typhimurium cells have been performed using electrochemical impedance spectroscopy (EIS) technique. The fabricated microfluidic immunosensor offers highly improved biosensing parameters, as sensitivity was found to be 1.79 kΩ/CFU−1mL cm−2 with the detection limit 1.56 CFU mL−1in the wide detection range of 101–107 CFUmL−1.
KW - Conductivity
KW - Functionalized 2D MoS nanosheets
KW - Immunosensor
KW - Microfluidics
KW - Salmonella typhimurium
KW - Sensitivity
UR - http://www.scopus.com/inward/record.url?scp=85039773628&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2017.12.094
DO - 10.1016/j.snb.2017.12.094
M3 - Article
AN - SCOPUS:85039773628
SN - 0925-4005
VL - 259
SP - 1090
EP - 1098
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -