Functionally coated polyethersulfone hollow fiber membranes: A substrate for enhanced HepG2/C3A functions

Surendra Kumar Verma, Akshay Modi, Atul Kumar Singh, Rohit Teotia, Sachin Kadam, Jayesh Bellare

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Hollow fiber membrane (HFM) based liver assist systems are a life-saving bridge for patients until a donor organ is available for transplantation or until liver regeneration. However, liver cell attachment and functional maintenance on HFM surface is a major challenge in bio-artificial liver (BAL) support systems. In the present study, novel glutaraldehyde (GTA)-crosslinked gelatin (gel)-coated polyethersulfone (X-gel-PT) HFMs were manufactured using triple orifice spinneret by the dry-wet spinning method. HFMs were characterized for morphology, outer surface roughness, hydrophilicity, tensile strength, thermal stability, BET surface area and pore volume measurements, permeability and rejection. Fourier transform infrared spectroscopy, and transmission electron microscopy confirmed the GTA-crosslinked gel-coating in the X-gel-PT HFMs, which provided the desirable extracellular matrix-like environment to the HepG2/C3A cells. The results of in-vitro hemocompatibility tests showed the better suitability of the developed HFMs for the blood-contact application. X-gel-PT HFMs showed significantly better cellular attachment and proliferation of HepG2/C3A cells on day 3 and 6, as shown by scanning electron and confocal microscopy. Significantly high urea synthesis and albumin secretion seen indicated the improved functional and metabolic activity of HepG2/C3A cells. Thus, the developed X-gel-PT HFMs is a suitable substrate for the hepatocyte culture, mass culture, and development of BAL support system.

Original languageEnglish
Pages (from-to)358-369
Number of pages12
JournalColloids and Surfaces B: Biointerfaces
StatePublished - 1 Apr 2018
Externally publishedYes


  • Bio-artificial liver
  • Crosslinking
  • HepG2/C3A cells
  • Hollow fiber membrane
  • Polyethersulfone
  • Triple orifice coating

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry


Dive into the research topics of 'Functionally coated polyethersulfone hollow fiber membranes: A substrate for enhanced HepG2/C3A functions'. Together they form a unique fingerprint.

Cite this