TY - JOUR
T1 - Functionally coated polyethersulfone hollow fiber membranes
T2 - A substrate for enhanced HepG2/C3A functions
AU - Verma, Surendra Kumar
AU - Modi, Akshay
AU - Singh, Atul Kumar
AU - Teotia, Rohit
AU - Kadam, Sachin
AU - Bellare, Jayesh
N1 - Funding Information:
The authors are thankful to Centre for Research in Nanotechnology and Science (CRNTS), Department of Chemical Engineering, Department of Chemistry, Department of Metallurgical Engineering and Materials Science, and Central Instrumentation Facility, Indian Institute of Technology (IIT) Bombay, India for providing the access to various characterization facilities used in this study. The authors acknowledge research fellowship grants from the U.G.C., India (S.K.V.), M.H.R.D., Government of India (A.M. & R.T.) and DST-INSPIRE, Government of India (A.K.S.). The authors acknowledge the research grant received from DBT, India (BT/PR3138/NNT/28/550/2011). The authors also acknowledge the Wadhwani Research Centre for Bioengineering, IIT Bombay, India for the research grants.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Hollow fiber membrane (HFM) based liver assist systems are a life-saving bridge for patients until a donor organ is available for transplantation or until liver regeneration. However, liver cell attachment and functional maintenance on HFM surface is a major challenge in bio-artificial liver (BAL) support systems. In the present study, novel glutaraldehyde (GTA)-crosslinked gelatin (gel)-coated polyethersulfone (X-gel-PT) HFMs were manufactured using triple orifice spinneret by the dry-wet spinning method. HFMs were characterized for morphology, outer surface roughness, hydrophilicity, tensile strength, thermal stability, BET surface area and pore volume measurements, permeability and rejection. Fourier transform infrared spectroscopy, and transmission electron microscopy confirmed the GTA-crosslinked gel-coating in the X-gel-PT HFMs, which provided the desirable extracellular matrix-like environment to the HepG2/C3A cells. The results of in-vitro hemocompatibility tests showed the better suitability of the developed HFMs for the blood-contact application. X-gel-PT HFMs showed significantly better cellular attachment and proliferation of HepG2/C3A cells on day 3 and 6, as shown by scanning electron and confocal microscopy. Significantly high urea synthesis and albumin secretion seen indicated the improved functional and metabolic activity of HepG2/C3A cells. Thus, the developed X-gel-PT HFMs is a suitable substrate for the hepatocyte culture, mass culture, and development of BAL support system.
AB - Hollow fiber membrane (HFM) based liver assist systems are a life-saving bridge for patients until a donor organ is available for transplantation or until liver regeneration. However, liver cell attachment and functional maintenance on HFM surface is a major challenge in bio-artificial liver (BAL) support systems. In the present study, novel glutaraldehyde (GTA)-crosslinked gelatin (gel)-coated polyethersulfone (X-gel-PT) HFMs were manufactured using triple orifice spinneret by the dry-wet spinning method. HFMs were characterized for morphology, outer surface roughness, hydrophilicity, tensile strength, thermal stability, BET surface area and pore volume measurements, permeability and rejection. Fourier transform infrared spectroscopy, and transmission electron microscopy confirmed the GTA-crosslinked gel-coating in the X-gel-PT HFMs, which provided the desirable extracellular matrix-like environment to the HepG2/C3A cells. The results of in-vitro hemocompatibility tests showed the better suitability of the developed HFMs for the blood-contact application. X-gel-PT HFMs showed significantly better cellular attachment and proliferation of HepG2/C3A cells on day 3 and 6, as shown by scanning electron and confocal microscopy. Significantly high urea synthesis and albumin secretion seen indicated the improved functional and metabolic activity of HepG2/C3A cells. Thus, the developed X-gel-PT HFMs is a suitable substrate for the hepatocyte culture, mass culture, and development of BAL support system.
KW - Bio-artificial liver
KW - Crosslinking
KW - HepG2/C3A cells
KW - Hollow fiber membrane
KW - Polyethersulfone
KW - Triple orifice coating
UR - http://www.scopus.com/inward/record.url?scp=85041459717&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2018.01.038
DO - 10.1016/j.colsurfb.2018.01.038
M3 - Article
C2 - 29413617
AN - SCOPUS:85041459717
SN - 0927-7765
VL - 164
SP - 358
EP - 369
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -