G-protein-coupled receptors function as logic gates for nanoparticle binding using systems and synthetic biology approach

Aman Chandra Kaushik, Xueying Mao, Cheng Dong Li, Yan Li, Dong Qing Wei, Shakti Sahi

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

G-protein-coupled receptor 142 (GPR142) belongs to rhodopsin family. GPR142 and GPR119, both Gq-coupled receptors, are expressed in pancreatic β cells of pancreas; their activation eventually leads to triggering of insulin secretion. In this paper, through a systems and synthetic biology approach, the effect of a common hit compound has been investigated in GPR142 and GPR119 pathways. This hit that has the potential to be developed as a lead for nanodrug was obtained through high-Throughput virtual screening. The hit compound was further docked with nanoparticles (GOLD, SPION, and CeO2). The probable effect of this potential hit on insulin secretion in type 2 diabetes and its dynamic behavior was explored. Kinetic simulation was performed for cross-validation of its role in both the pathways. This study opens up a probable avenue in therapy of type 2 diabetes through regulation of GPR142 and GPR119 receptors. The biological circuit constructed may further have an application as a modulator to control the up-and downregulation of the biochemical pathway and can be implemented as sensors or nanochips for therapy.

Original languageEnglish
Pages (from-to)1854-1867
Number of pages14
JournalJournal of Materials Research
Volume34
Issue number11
DOIs
StatePublished - 14 Jun 2019
Externally publishedYes

Keywords

  • GPR119
  • GPR142
  • nanodrug
  • nanoparticle
  • synthetic biology
  • systems biology
  • type 2 diabetes

ASJC Scopus subject areas

  • Materials Science (all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Fingerprint

Dive into the research topics of 'G-protein-coupled receptors function as logic gates for nanoparticle binding using systems and synthetic biology approach'. Together they form a unique fingerprint.

Cite this