TY - JOUR
T1 - GABA shunt deficiencies and accumulation of reactive oxygen intermediates
T2 - Insight from Arabidopsis mutants
AU - Fait, Aaron
AU - Yellin, Ayelet
AU - Fromm, Hillel
N1 - Funding Information:
We thank Prof. Gad Galili for continuous support and helpful discussion, and Janiv Semel for helpful discussion and assistance in chromatographic data elaboration. GHB was a kind gift from Dr. Zafrir Goren, Chief Superintendent, Israel Police Force.
PY - 2005/1/17
Y1 - 2005/1/17
N2 - In plants, succinic semialdehyde dehydrogenase (SSADH)-deficiency results in the accumulation of reactive oxygen intermediates (ROI), necrotic lesions, dwarfism, and hypersensitivity to environmental stresses [Bouché, N., Fait, A., Moller, S.G. and Fromm, H. (2003) Proc. Natl. Acad. Sci. USA. 100, 6843-6848]. We report that Arabidopsis ssadh knockout mutants contain five times the normal level of γ-hydroxybutyrate (GHB), which in SSADH-deficient mammals accounts for phenotypic abnormalities. Moreover, the level of GHB in Arabidopsis is light dependent. Treatment with γ-vinyl-γ- aminobutyrate, a specific γ-aminobutyrate (GABA)-transaminase inhibitor, prevents the accumulation of ROI and GHB in ssadh mutants, inhibits cell death, and improves growth. These results provide novel evidence for the relationship between the GABA shunt and ROI, which may, in part, explain the phenotype of SSADH-deficient plants and animals.
AB - In plants, succinic semialdehyde dehydrogenase (SSADH)-deficiency results in the accumulation of reactive oxygen intermediates (ROI), necrotic lesions, dwarfism, and hypersensitivity to environmental stresses [Bouché, N., Fait, A., Moller, S.G. and Fromm, H. (2003) Proc. Natl. Acad. Sci. USA. 100, 6843-6848]. We report that Arabidopsis ssadh knockout mutants contain five times the normal level of γ-hydroxybutyrate (GHB), which in SSADH-deficient mammals accounts for phenotypic abnormalities. Moreover, the level of GHB in Arabidopsis is light dependent. Treatment with γ-vinyl-γ- aminobutyrate, a specific γ-aminobutyrate (GABA)-transaminase inhibitor, prevents the accumulation of ROI and GHB in ssadh mutants, inhibits cell death, and improves growth. These results provide novel evidence for the relationship between the GABA shunt and ROI, which may, in part, explain the phenotype of SSADH-deficient plants and animals.
KW - Arabidopsis
KW - Reactive oxygen intermediate
KW - Succinic semialdehyde dehydrogenase
KW - γ-Aminobutyrate
KW - γ-Hydroxybutyrate
UR - http://www.scopus.com/inward/record.url?scp=11844252078&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2004.12.004
DO - 10.1016/j.febslet.2004.12.004
M3 - Article
C2 - 15642352
AN - SCOPUS:11844252078
SN - 0014-5793
VL - 579
SP - 415
EP - 420
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -