Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype

Ran Rostoker, Hiba Yaseen, Sagie Schif-Zuck, Rachel G. Lichtenstein, Gabriel A. Rabinovich, Amiram Ariel

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11b low macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalProstaglandins and Other Lipid Mediators
Volume107
DOIs
StatePublished - 3 Sep 2013

Keywords

  • 15-Lipoxygenase
  • Efferocytosis
  • Galectin-1
  • Macrophages
  • Resolution of inflammation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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