TY - JOUR
T1 - Gas chromatographic assay with pharmacokinetic applications for monitoring T-2 and HT-2 toxins in plasma
AU - Yagen, Boris
AU - Bialer, Meir
AU - Sintov, Amnon
N1 - Funding Information:
This paper is taken from the doctoral dissertation of Mr. A. Sintov as partial fulfillment of the PhD requirements of the Hebrew University of Jerusalem. This work was supported by a grant from the Chief Scientist office of the Israel Ministry of Health and the Israel National Council for Research and Development.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - A gas-liquid chromatographic (GLC) method for monitoring T-2 and HT-2 toxins in plasma was developed. The procedure involved extraction of the toxins with ethyl acetate, chromatography on a C18 reversed-phase column and derivatization with heptafluorobutyric anhydride (HFBA). The T-2 and HT-2 HFBA derivatives were chromatographed on OV-17 at various temperatures and measured with an electron-capture detector. Iso-T-2 toxin and iso-HT-2 toxin were used as internal standards. Recoveries averaged 95.1 ± 8.6% for T-2 toxin and 102.1 ± 5.2% for HT-2 toxin at levels ranging from 40 to 120 ng/ml. The limits of detection were 30 and 5 ng/ml of T-2 and HT-2 toxin, respectively. The range of the assay covers plasma concentrations at which toxicity becomes manifest. The pharmacokinetic application of this GLC method is illustrated by simultaneous monitoring of T-2 and HT-2 toxins levels in plasma obtained after intravenous administration of T-2 toxin to a dog.
AB - A gas-liquid chromatographic (GLC) method for monitoring T-2 and HT-2 toxins in plasma was developed. The procedure involved extraction of the toxins with ethyl acetate, chromatography on a C18 reversed-phase column and derivatization with heptafluorobutyric anhydride (HFBA). The T-2 and HT-2 HFBA derivatives were chromatographed on OV-17 at various temperatures and measured with an electron-capture detector. Iso-T-2 toxin and iso-HT-2 toxin were used as internal standards. Recoveries averaged 95.1 ± 8.6% for T-2 toxin and 102.1 ± 5.2% for HT-2 toxin at levels ranging from 40 to 120 ng/ml. The limits of detection were 30 and 5 ng/ml of T-2 and HT-2 toxin, respectively. The range of the assay covers plasma concentrations at which toxicity becomes manifest. The pharmacokinetic application of this GLC method is illustrated by simultaneous monitoring of T-2 and HT-2 toxins levels in plasma obtained after intravenous administration of T-2 toxin to a dog.
UR - http://www.scopus.com/inward/record.url?scp=0022357864&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)84569-7
DO - 10.1016/S0378-4347(00)84569-7
M3 - Article
AN - SCOPUS:0022357864
SN - 1387-2273
VL - 343
SP - 67
EP - 75
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -