Gas chromatographic assay with pharmacokinetic applications for monitoring T-2 and HT-2 toxins in plasma

Boris Yagen, Meir Bialer, Amnon Sintov

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12 Scopus citations

Abstract

A gas-liquid chromatographic (GLC) method for monitoring T-2 and HT-2 toxins in plasma was developed. The procedure involved extraction of the toxins with ethyl acetate, chromatography on a C18 reversed-phase column and derivatization with heptafluorobutyric anhydride (HFBA). The T-2 and HT-2 HFBA derivatives were chromatographed on OV-17 at various temperatures and measured with an electron-capture detector. Iso-T-2 toxin and iso-HT-2 toxin were used as internal standards. Recoveries averaged 95.1 ± 8.6% for T-2 toxin and 102.1 ± 5.2% for HT-2 toxin at levels ranging from 40 to 120 ng/ml. The limits of detection were 30 and 5 ng/ml of T-2 and HT-2 toxin, respectively. The range of the assay covers plasma concentrations at which toxicity becomes manifest. The pharmacokinetic application of this GLC method is illustrated by simultaneous monitoring of T-2 and HT-2 toxins levels in plasma obtained after intravenous administration of T-2 toxin to a dog.

Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume343
Issue numberC
DOIs
StatePublished - 1 Jan 1985
Externally publishedYes

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