Gene expression microarray profiles of cumulus cells in lean and overweight-obese polycystic ovary syndrome patients

Shlomit Kenigsberg, Yaakov Bentov, Vered Chalifa-Caspi, Gad Potashnik, Rivka Ofir, Ohad S. Birk

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

The aim of this work was to study gene expression patterns of cultured cumulus cells from lean and overweight-obese polycystic ovary syndrome (PCOS) patients using genome-wide oligonucleotide microarray. The study included 25 patients undergoing in vitro fertilization and intra-cytoplasmic sperm injection: 12 diagnosed with PCOS and 13 matching controls. Each of the groups was subdivided into lean (body mass index (BMI) < 24) and overweight (BMI > 27) subgroups. The following comparisons of gene expression data were made: lean PCOS versus lean controls, lean PCOS versus overweight PCOS, all PCOS versus all controls, overweight PCOS versus overweight controls, overweight controls versus lean controls and all overweight versus all lean. The largest number of differentially expressed genes (DEGs), with fold change (FC)/FC/≥ 1.5 and P-value < 0.01, was found in the lean PCOS versus lean controls comparison (487) with most of these genes being down-regulated in PCOS. The second largest group of DEGs originated from the comparison of lean PCOS versus overweight PCOS (305). The other comparisons resulted in a much smaller number of DEGs (174, 109, 125 and 12, respectively). In the comparison of lean PCOS with lean controls, most DEGs were transcription factors and components of the extracellular matrix and two pathways, Wnt/β-catenin and mitogen-activated protein kinase. When comparing overweight PCOS with overweight controls, most DEGs were of pathways related to insulin signaling, metabolism and energy production. The finding of unique gene expression patterns in cumulus cells from the two PCOS subtypes is in agreement with other studies that have found the two to be separate entities with potentially different pathophysiologies.

Original languageEnglish
Pages (from-to)89-103
Number of pages15
JournalMolecular Human Reproduction
Volume15
Issue number2
DOIs
StatePublished - 19 Feb 2009

Keywords

  • Cumulus
  • Infertility
  • Insulin resistance
  • Microarray
  • PCOS

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Gene expression microarray profiles of cumulus cells in lean and overweight-obese polycystic ovary syndrome patients'. Together they form a unique fingerprint.

Cite this