Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries

Mila Ayash-Rashkovsky; Ziva Weisman; Jocelyn Diveley; Ronald B. Moss; Zvi Bentwich; Gadi Borkow

doi:10.1016/S0264-410X(02)00202-5

Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries

Mila Ayash-Rashkovsky, Ziva Weisman, Jocelyn Diveley, Ronald B. Moss, Zvi Bentwich, Gadi Borkow

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Vaccination against HIV-1 of hosts with a dominant Th2 immune profile may fail to induce essential protective Th1 immune responses. By using Schistosoma-infected mice, with a pre-existent Th2 immune background, we demonstrate that oligodeoxynucleotides (ODN) containing unmethylated cytosine-phosphate-guanosine (CpG) immunostimulatory sequences co-administered with inactivated, gp120-depleted HIV-1 viral particles (HIV-1 immunogen) lead to potent Th1 anti-HIV-1 immune responses overcoming the Th2 bias. In contrast, Schistosoma-infected mice immunized with HIV-1 immunogen in incomplete Freund's adjuvant only, induced Th2 anti-HIV-1 immune responses. These findings strongly support the advisability of using CpG ODN as a Th1 inducing adjuvant when immunizing human populations with a strong pre-existent Th2 immune profile.

Original language	English
Pages (from-to)	2684-2692
Number of pages	9
Journal	Vaccine
Volume	20
Issue number	21-22
DOIs	https://doi.org/10.1016/S0264-410X(02)00202-5
State	Published - 21 Jun 2002
Externally published	Yes

Keywords

CpG ODN
HIV-1 immunogen
Schistosoma
Th1 and Th2 cytokine profile

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology (all)
Veterinary (all)
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(02)00202-5

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Ayash-Rashkovsky, M., Weisman, Z., Diveley, J., Moss, R. B., Bentwich, Z., & Borkow, G. (2002). Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries. Vaccine, 20(21-22), 2684-2692. https://doi.org/10.1016/S0264-410X(02)00202-5

@article{dd673d2fb785406baa54eb5393270cac,

title = "Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries",

abstract = "Vaccination against HIV-1 of hosts with a dominant Th2 immune profile may fail to induce essential protective Th1 immune responses. By using Schistosoma-infected mice, with a pre-existent Th2 immune background, we demonstrate that oligodeoxynucleotides (ODN) containing unmethylated cytosine-phosphate-guanosine (CpG) immunostimulatory sequences co-administered with inactivated, gp120-depleted HIV-1 viral particles (HIV-1 immunogen) lead to potent Th1 anti-HIV-1 immune responses overcoming the Th2 bias. In contrast, Schistosoma-infected mice immunized with HIV-1 immunogen in incomplete Freund's adjuvant only, induced Th2 anti-HIV-1 immune responses. These findings strongly support the advisability of using CpG ODN as a Th1 inducing adjuvant when immunizing human populations with a strong pre-existent Th2 immune profile.",

keywords = "CpG ODN, HIV-1 immunogen, Schistosoma, Th1 and Th2 cytokine profile",

author = "Mila Ayash-Rashkovsky and Ziva Weisman and Jocelyn Diveley and Moss, {Ronald B.} and Zvi Bentwich and Gadi Borkow",

note = "Funding Information: This work is dedicated to Ziva Weisman, a co-author of this study, who has passed away. The work was supported by the Horowitz Foundation. We thank Rebecca Tarrab-Hazdai for her support, helpful discussions, and for providing us with Schistosoma -infected mice, Schistosoma cercaria and adult worm antigens. ",

year = "2002",

month = jun,

day = "21",

doi = "10.1016/S0264-410X(02)00202-5",

language = "English",

volume = "20",

pages = "2684--2692",

journal = "Vaccine",

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Ayash-Rashkovsky, M, Weisman, Z, Diveley, J, Moss, RB, Bentwich, Z & Borkow, G 2002, 'Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries', Vaccine, vol. 20, no. 21-22, pp. 2684-2692. https://doi.org/10.1016/S0264-410X(02)00202-5

Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries. / Ayash-Rashkovsky, Mila; Weisman, Ziva; Diveley, Jocelyn et al.
In: Vaccine, Vol. 20, No. 21-22, 21.06.2002, p. 2684-2692.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries

AU - Ayash-Rashkovsky, Mila

AU - Weisman, Ziva

AU - Diveley, Jocelyn

AU - Moss, Ronald B.

AU - Bentwich, Zvi

AU - Borkow, Gadi

N1 - Funding Information: This work is dedicated to Ziva Weisman, a co-author of this study, who has passed away. The work was supported by the Horowitz Foundation. We thank Rebecca Tarrab-Hazdai for her support, helpful discussions, and for providing us with Schistosoma -infected mice, Schistosoma cercaria and adult worm antigens.

PY - 2002/6/21

Y1 - 2002/6/21

N2 - Vaccination against HIV-1 of hosts with a dominant Th2 immune profile may fail to induce essential protective Th1 immune responses. By using Schistosoma-infected mice, with a pre-existent Th2 immune background, we demonstrate that oligodeoxynucleotides (ODN) containing unmethylated cytosine-phosphate-guanosine (CpG) immunostimulatory sequences co-administered with inactivated, gp120-depleted HIV-1 viral particles (HIV-1 immunogen) lead to potent Th1 anti-HIV-1 immune responses overcoming the Th2 bias. In contrast, Schistosoma-infected mice immunized with HIV-1 immunogen in incomplete Freund's adjuvant only, induced Th2 anti-HIV-1 immune responses. These findings strongly support the advisability of using CpG ODN as a Th1 inducing adjuvant when immunizing human populations with a strong pre-existent Th2 immune profile.

AB - Vaccination against HIV-1 of hosts with a dominant Th2 immune profile may fail to induce essential protective Th1 immune responses. By using Schistosoma-infected mice, with a pre-existent Th2 immune background, we demonstrate that oligodeoxynucleotides (ODN) containing unmethylated cytosine-phosphate-guanosine (CpG) immunostimulatory sequences co-administered with inactivated, gp120-depleted HIV-1 viral particles (HIV-1 immunogen) lead to potent Th1 anti-HIV-1 immune responses overcoming the Th2 bias. In contrast, Schistosoma-infected mice immunized with HIV-1 immunogen in incomplete Freund's adjuvant only, induced Th2 anti-HIV-1 immune responses. These findings strongly support the advisability of using CpG ODN as a Th1 inducing adjuvant when immunizing human populations with a strong pre-existent Th2 immune profile.

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KW - Schistosoma

KW - Th1 and Th2 cytokine profile

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DO - 10.1016/S0264-410X(02)00202-5

M3 - Article

C2 - 12034094

AN - SCOPUS:0037150759

SN - 0264-410X

VL - 20

SP - 2684

EP - 2692

JO - Vaccine

JF - Vaccine

IS - 21-22

ER -

Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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