Generation of Th1 immune responses to inactivated, gp120-depleted HIV-1 in mice with a dominant Th2 biased immune profile via imunostimulatory oligonucleotides - Relevance to AIDS vaccines in developing countries

Mila Ayash-Rashkovsky, Ziva Weisman, Jocelyn Diveley, Ronald B. Moss, Zvi Bentwich, Gadi Borkow

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Vaccination against HIV-1 of hosts with a dominant Th2 immune profile may fail to induce essential protective Th1 immune responses. By using Schistosoma-infected mice, with a pre-existent Th2 immune background, we demonstrate that oligodeoxynucleotides (ODN) containing unmethylated cytosine-phosphate-guanosine (CpG) immunostimulatory sequences co-administered with inactivated, gp120-depleted HIV-1 viral particles (HIV-1 immunogen) lead to potent Th1 anti-HIV-1 immune responses overcoming the Th2 bias. In contrast, Schistosoma-infected mice immunized with HIV-1 immunogen in incomplete Freund's adjuvant only, induced Th2 anti-HIV-1 immune responses. These findings strongly support the advisability of using CpG ODN as a Th1 inducing adjuvant when immunizing human populations with a strong pre-existent Th2 immune profile.

Original languageEnglish
Pages (from-to)2684-2692
Number of pages9
JournalVaccine
Volume20
Issue number21-22
DOIs
StatePublished - 21 Jun 2002
Externally publishedYes

Keywords

  • CpG ODN
  • HIV-1 immunogen
  • Schistosoma
  • Th1 and Th2 cytokine profile

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