Genetic analysis of generation of serum interferon by bacterial lipopolysaccharide

R. N. Apte, O. Ascher, D. H. Pluznik

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Administration of bacterial lipopolysaccharides (LPS) to mice causes interferon (IF) to appear in their blood stream. The generation of this serum IF is 'early' and its maximum concentration appears 2 hr after an inoculation of a single optimal dose of LPS (100 μg). Two inbread strains of mice differing in their ability to generate LPS-induced serum IF were used: a high responder strain (C3H/eB), which generates high levels of IF (about 100 IF units/ml serum), and a low responder strain (C3H/HeJ), which fails to generate any detectable amounts of IF in the serum. A genetic analysis was carried out and circulating IF production was determined in the F1 hybrid, in the F2 generation, and in the progeny of backcrosses of F1 hybrids to either parent strain. The results indicate that a single dominant autosomal gene is responsible for the difference in circulating IF production between both parent strains. In addition it was shown that this type of IF is stable to treatment at pH 2 for periods of time as long as 48 hr, but is labile to heating at 56°C for 30 min.

Original languageEnglish
Pages (from-to)1898-1902
Number of pages5
JournalJournal of Immunology
Issue number6
StatePublished - 1 Dec 1977
Externally publishedYes


Dive into the research topics of 'Genetic analysis of generation of serum interferon by bacterial lipopolysaccharide'. Together they form a unique fingerprint.

Cite this