Genetic Code Expansion of Vibrio natriegens

Eden Ozer, Lital Alfonta

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Escherichia coli has been considered as the most used model bacteria in the majority of studies for several decades. However, a new, faster chassis for synthetic biology is emerging in the form of the fast-growing gram-negative bacterium Vibrio natriegens. Different methodologies, well established in E. coli, are currently being adapted for V. natriegens in the hope to enable a much faster platform for general molecular biology studies. Amongst the vast technologies available for E. coli, genetic code expansion, the incorporation of unnatural amino acids into proteins, serves as a robust tool for protein engineering and biorthogonal modifications. Here we designed and adapted the genetic code expansion methodology for V. natriegens and demonstrate an unnatural amino acid incorporation into a protein for the first time in this organism.

Original languageEnglish
Article number594429
JournalFrontiers in Bioengineering and Biotechnology
StatePublished - 26 Feb 2021


  • Vibrio natriegens
  • genetic code expansion
  • propargyl-L-lysine
  • synthetic biology
  • unnatural amino acids

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering


Dive into the research topics of 'Genetic Code Expansion of Vibrio natriegens'. Together they form a unique fingerprint.

Cite this