Genetic relationships among Mediterranean Pistacia species evaluated by RAPD and AFLP markers

A. Golan-Goldhirsh, O. Barazani, Z. S. Wang, D. K. Khadka, J. A. Saunders, V. Kostiukovsky, L. J. Rowland

    Research output: Contribution to journalArticlepeer-review

    54 Scopus citations

    Abstract

    Polymorphisms among Mediterranean basin Pistacia species and accessions within species were assessed by random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) analyses. Twenty-eight Pistacia accessions representing six species from geographically diverse locations in the Mediterranean area were analyzed. With RAPD, a total of 259 DNA fragments were amplified by 27 preselected primers, 254 were polymorphic fragments. AFLP analysis with 15 primer sets, produced 954 (93%) polymorphic bands out of a total of 1026. A Mantel test revealed an extremely high correlation (r = 0.99) between similarity matrices generated from RAPD and AFLP data sets, indicating that similar results were obtained by the two techniques. Dendrograms constructed from the similarity matrices showed that Pistacia species could be clustered into two groups, one group containing all the P. lentiscus and the second group containing all other accessions. The latter group was divided into two subgroups, one consisting of P. palaestina and P. terebinthus; the other consisting of P. atlantica, P. khinjuk and P. vera. P. vera and P. khinjuk were highly similar, as were P. palaestina and P. terebinthus.

    Original languageEnglish
    Pages (from-to)9-18
    Number of pages10
    JournalPlant Systematics and Evolution
    Volume246
    Issue number1-2
    DOIs
    StatePublished - 1 Jun 2004

    Keywords

    • AFLP
    • Genetic relationship
    • Germplasm
    • Molecular marker
    • Pistacia spp.
    • Polymorphism
    • RAPD

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Plant Science

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