TY - JOUR
T1 - Genetic variability in Turkmen populations of Pistacia vera L.
AU - Barazani, Oz
AU - Atayev, Azat
AU - Yakubov, Bakhtiyor
AU - Kostiukovsky, Vladimir
AU - Popov, Konstantin
AU - Golan-Goldhirsh, Avi
N1 - Funding Information:
We thank Drs M. Nepesov, B. Mamedov and M. Durikov for their cooperative assistance in seed collection and organization of field expeditions and for the able translator function of Dr Lea Orlovsky. The guidance of Mr Taganduri Igshi Muradov in the Badghyz reserve is greatly appreciated. This research was supported by AID-CDR Grant No. TA-MOU-98-CA17-028
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Seedlings of Pistacia vera L. developed from seeds of two separate populations in Turkmenistan, Kepele and Agachli, were evaluated for their growth potential and genetic polymorphism. Plant growth rate as well as random amplified polymorphic DNA (RAPD) analysis showed distinct differences between the two populations. In plant height growth rate, 17 Agachli accessions were 1.3 times higher on average than that of 10 accessions of Kepele (significant at p = 0.046) and 1.2 times higher for trunk diameter growth rate (p = 0.062). Cluster analysis divided most accessions into two main genetic groups according to their geographic origin. The Agachli group was further divided into two subgroups. One Kepele accession (K4), was genetically different from the rest and clustered on a separate outgroup. Two Agachli accessions (A12 and A17) were outside the two main populations clusters. Accessions K9 and K10 from Kepele were exceptional and were clustered in each of the two Agachli's subgroups, indicating a close genetic relationship between the two populations. In addition, high similarity values (0.58-1.00) and small genetic distances reflect plausible gene flow between Kepele and Agachli, which are 100 Km apart. Mantel test revealed significant relationship between the RAPD and the morphological traits matrices, pointing to the genetic basis for the measured differences in the growth rate. Growing the accessions on the same plot, under similar conditions enabled the evaluation of genotypic differences. The combination of morphological traits and molecular markers will further assist in preservation of genetic variability and cultivation of useful genotypes of P. vera L.
AB - Seedlings of Pistacia vera L. developed from seeds of two separate populations in Turkmenistan, Kepele and Agachli, were evaluated for their growth potential and genetic polymorphism. Plant growth rate as well as random amplified polymorphic DNA (RAPD) analysis showed distinct differences between the two populations. In plant height growth rate, 17 Agachli accessions were 1.3 times higher on average than that of 10 accessions of Kepele (significant at p = 0.046) and 1.2 times higher for trunk diameter growth rate (p = 0.062). Cluster analysis divided most accessions into two main genetic groups according to their geographic origin. The Agachli group was further divided into two subgroups. One Kepele accession (K4), was genetically different from the rest and clustered on a separate outgroup. Two Agachli accessions (A12 and A17) were outside the two main populations clusters. Accessions K9 and K10 from Kepele were exceptional and were clustered in each of the two Agachli's subgroups, indicating a close genetic relationship between the two populations. In addition, high similarity values (0.58-1.00) and small genetic distances reflect plausible gene flow between Kepele and Agachli, which are 100 Km apart. Mantel test revealed significant relationship between the RAPD and the morphological traits matrices, pointing to the genetic basis for the measured differences in the growth rate. Growing the accessions on the same plot, under similar conditions enabled the evaluation of genotypic differences. The combination of morphological traits and molecular markers will further assist in preservation of genetic variability and cultivation of useful genotypes of P. vera L.
KW - Genetic variability
KW - Morphology
KW - Pistacia vera L.
KW - Polymorphism
KW - RAPD
KW - Turkmenistan
UR - http://www.scopus.com/inward/record.url?scp=0037704259&partnerID=8YFLogxK
U2 - 10.1023/A:1023928017410
DO - 10.1023/A:1023928017410
M3 - Article
AN - SCOPUS:0037704259
SN - 0925-9864
VL - 50
SP - 383
EP - 389
JO - Genetic Resources and Crop Evolution
JF - Genetic Resources and Crop Evolution
IS - 4
ER -
