Genetically encoded optical activation of DNA recombination in human cells

J. Luo, E. Arbely, J. Zhang, C. Chou, R. Uprety, J. W. Chin, A. Deiters

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

Original languageEnglish
Pages (from-to)8529-8532
Number of pages4
JournalChemical Communications
Volume52
Issue number55
DOIs
StatePublished - 1 Jan 2016

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • General Chemistry
  • Catalysis

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