Genetically encoded optical activation of DNA recombination in human cells

J. Luo; E. Arbely; J. Zhang; C. Chou; R. Uprety; J. W. Chin; A. Deiters

doi:10.1039/c6cc03934k

Genetically encoded optical activation of DNA recombination in human cells

J. Luo, E. Arbely, J. Zhang, C. Chou, R. Uprety, J. W. Chin, A. Deiters

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

Original language	English
Pages (from-to)	8529-8532
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	55
DOIs	https://doi.org/10.1039/c6cc03934k
State	Published - 1 Jan 2016

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "Genetically encoded optical activation of DNA recombination in human cells",

abstract = "We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.",

author = "J. Luo and E. Arbely and J. Zhang and C. Chou and R. Uprety and Chin, \{J. W.\} and A. Deiters",

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T1 - Genetically encoded optical activation of DNA recombination in human cells

AU - Luo, J.

AU - Arbely, E.

AU - Zhang, J.

AU - Chou, C.

AU - Uprety, R.

AU - Chin, J. W.

AU - Deiters, A.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

AB - We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.

UR - https://www.scopus.com/pages/publications/84977277853

U2 - 10.1039/c6cc03934k

DO - 10.1039/c6cc03934k

M3 - Article

C2 - 27277957

AN - SCOPUS:84977277853

SN - 1359-7345

VL - 52

SP - 8529

EP - 8532

JO - Chemical Communications

JF - Chemical Communications

IS - 55

ER -

Genetically encoded optical activation of DNA recombination in human cells

Abstract

ASJC Scopus subject areas

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