TY - JOUR
T1 - Genome editing technologies, mechanisms and improved production of therapeutic phytochemicals
T2 - Opportunities and prospects
AU - Mitra, Sicon
AU - Anand, Uttpal
AU - Ghorai, Mimosa
AU - Kant, Nishi
AU - Kumar, Manoj
AU - Radha,
AU - Jha, Niraj K.
AU - Swamy, Mallappa K.
AU - Proćków, Jarosław
AU - de la Lastra, José M.Pérez
AU - Dey, Abhijit
N1 - Publisher Copyright:
© 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Plants produce a large number of secondary metabolites, known as phytometabolites that may be employed as medicines, dyes, poisons, and insecticides in the field of medicine, agriculture, and industrial use, respectively. The rise of genome management approaches has promised a factual revolution in genetic engineering. Targeted genome editing in living entities permits the understanding of the biological systems very clearly, and also sanctions to address a wide-ranging objective in the direction of improving features of plant and their yields. The last few years have introduced a number of unique genome editing systems, including transcription activator-like effector nucleases, zinc finger nucleases, and miRNA-regulated clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). Genome editing systems have helped in the transformation of metabolic engineering, allowing researchers to modify biosynthetic pathways of different secondary metabolites. Given the growing relevance of editing genomes in plant research, the exciting novel methods are briefly reviewed in this chapter. Also, this chapter highlights recent discoveries on the CRISPR-based modification of natural products in different medicinal plants.
AB - Plants produce a large number of secondary metabolites, known as phytometabolites that may be employed as medicines, dyes, poisons, and insecticides in the field of medicine, agriculture, and industrial use, respectively. The rise of genome management approaches has promised a factual revolution in genetic engineering. Targeted genome editing in living entities permits the understanding of the biological systems very clearly, and also sanctions to address a wide-ranging objective in the direction of improving features of plant and their yields. The last few years have introduced a number of unique genome editing systems, including transcription activator-like effector nucleases, zinc finger nucleases, and miRNA-regulated clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9). Genome editing systems have helped in the transformation of metabolic engineering, allowing researchers to modify biosynthetic pathways of different secondary metabolites. Given the growing relevance of editing genomes in plant research, the exciting novel methods are briefly reviewed in this chapter. Also, this chapter highlights recent discoveries on the CRISPR-based modification of natural products in different medicinal plants.
KW - CRISPR/Cas
KW - biosynthesis pathway
KW - gene encoding
KW - homozygous mutants
KW - knockout
KW - next-generation sequencing
UR - http://www.scopus.com/inward/record.url?scp=85140258570&partnerID=8YFLogxK
U2 - 10.1002/bit.28260
DO - 10.1002/bit.28260
M3 - Review article
C2 - 36224758
AN - SCOPUS:85140258570
SN - 0006-3592
VL - 120
SP - 82
EP - 94
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 1
ER -