Genome modifications in plant cells by custom-made restriction enzymes

Tzvi Tzfira, Dan Weinthal, Ira Marton, Vardit Zeevi, Amir Zuker, Alexander Vainstein

Research output: Contribution to journalReview articlepeer-review

48 Scopus citations

Abstract

Genome editing, i.e. the ability to mutagenize, insert, delete and replace sequences, in living cells is a powerful and highly desirable method that could potentially revolutionize plant basic research and applied biotechnology. Indeed, various research groups from academia and industry are in a race to devise methods and develop tools that will enable not only site-specific mutagenesis but also controlled foreign DNA integration and replacement of native and transgene sequences by foreign DNA, in living plant cells. In recent years, much of the progress seen in gene targeting in plant cells has been attributed to the development of zinc finger nucleases and other novel restriction enzymes for use as molecular DNA scissors. The induction of double-strand breaks at specific genomic locations by zinc finger nucleases and other novel restriction enzymes results in a wide variety of genetic changes, which range from gene addition to the replacement, deletion and site-specific mutagenesis of endogenous and heterologous genes in living plant cells. In this review, we discuss the principles and tools for restriction enzyme-mediated gene targeting in plant cells, as well as their current and prospective use for gene targeting in model and crop plants.

Original languageEnglish
Pages (from-to)373-389
Number of pages17
JournalPlant Biotechnology Journal
Volume10
Issue number4
DOIs
StatePublished - 1 May 2012

Keywords

  • DNA repair
  • Gene targeting
  • Genome editing
  • Genomic double strand breaks
  • TALE nucleases
  • Zinc finger nucleases

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