Genome-wide association of the metabolic shifts underpinning dark-induced senescence in Arabidopsis

Feng Zhu, Saleh Alseekh, Kaan Koper, Hao Tong, Zoran Nikoloski, Thomas Naake, Haijun Liu, Jianbing Yan, Yariv Brotman, Weiwei Wen, Hiroshi Maeda, Yunjiang Cheng, Alisdair R. Fernie

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Dark-induced senescence provokes profound metabolic shifts to recycle nutrients and to guarantee plant survival. To date, research on these processes has largely focused on characterizing mutants deficient in individual pathways. Here, we adopted a time-resolved genome-wide association-based approach to characterize dark-induced senescence by evaluating the photochemical efficiency and content of primary and lipid metabolites at the beginning, or after 3 or 6 days in darkness. We discovered six patterns of metabolic shifts and identified 215 associations with 81 candidate genes being involved in this process. Among these associations, we validated the roles of four genes associated with glycine, galactinol, threonine, and ornithine levels. We also demonstrated the function of threonine and galactinol catabolism during dark-induced senescence. Intriguingly, we determined that the association between tyrosine contents and TYROSINE AMINOTRANSFERASE 1 influences enzyme activity of the encoded protein and transcriptional activity of the gene under normal and dark conditions, respectively. Moreover, the single-nucleotide polymorphisms affecting the expression of THREONINE ALDOLASE 1 and the amino acid transporter gene AVT1B, respectively, only underlie the variation in threonine and glycine levels in the dark. Taken together, these results allow us to present a very detailed model of the metabolic aspects of dark-induced senescence, as well as the process itself.

Original languageEnglish
Pages (from-to)557-578
Number of pages22
JournalPlant Cell
Volume34
Issue number1
DOIs
StatePublished - 1 Jan 2022

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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