Genomic analysis of COP9 signalosome function in Drosophila melanogaster reveals a role in temporal regulation of gene expression

Efrat Oron, Tamir Tuller, Ling Li, Nina Rozovsky, Daniel Yekutieli, Sigal Rencus-Lazar, Daniel Segal, Benny Chor, Bruce A. Edgar, Daniel A. Chamovitz

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The COP9 signalosome (CSN), an eight-subunit protein complex, is conserved in all higher eukaryotes. CSN intersects the ubiquitin-proteasome pathway, modulating signaling pathways controlling various aspects of development. We are using Drosophila as a model system to elucidate the function of this important complex. Transcriptome data were generated for four csn mutants, sampled at three developmental time points. Our results are highly reproducible, being confirmed using two different experimental setups that entail different microarrays and different controls. Our results indicate that the CSN acts as a transcriptional repressor during development of Drosophila, resulting in achronic gene expression in the csn mutants. 'Time shift' analysis with the publicly available Drosophila transcriptome data indicates that genes repressed by the CSN are normally induced primarily during late embryogenesis or during metamorphosis. These temporal shifts are likely due to the roles of the CSN in regulating transcription factors. A null mutation in CSN subunit 4 and hypomorphic mutations in csn5 lead to more severe defects than seen in the csn5-null mutants strain, suggesting that CSN5 carries only some of the CSN function.

Original languageEnglish
JournalMolecular Systems Biology
Volume3
DOIs
StatePublished - 29 May 2007
Externally publishedYes

Keywords

  • COP9 signalosome
  • Drosophila
  • Heterochrony
  • JAB1
  • Transcriptome analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Immunology and Microbiology (all)
  • Agricultural and Biological Sciences (all)
  • Applied Mathematics

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