TY - JOUR
T1 - Genomic stability in reprogramming
AU - Ronen, Daniel
AU - Benvenisty, Nissim
N1 - Funding Information:
The authors would like to thank Tamar Golan-Lev for her assistance with the graphic design, and Uri Ben-David for critical reading of the manuscript. N.B. is the Herbert Cohn Chair in Cancer Research. This research was supported by The Legacy Heritage Biomedical Science Partnership Program of the Israel Science Foundation (grant no. 943/09 ) and by the Centers of Excellence Legacy Heritage Biomedical Science Partnership (grants no. 1801/10 ). D.R. is supported by the Lady Davis Fellowship Trust and the Israel Cancer Research Fund.
PY - 2012/10/1
Y1 - 2012/10/1
N2 - The genetic stability of induced pluripotent stem (iPS) cells has a significant impact on their potential use in regenerative medicine and basic research. Analysis of the genomic integrity of iPS cells suggests a tendency to develop aberrations ranging from whole chromosome trisomies to single nucleotide mutations. Furthermore, fluctuations in telomere elongation and changes in mitochondrial DNA are also observed. Some mutations may already exist in the founder cells or result from prolonged culturing, however, many of the mutations occur during the reprogramming event. Thus, great care should be given to the initial characterization and subsequent culturing of new iPS cell lines in order to avoid the use of potentially aberrant cells.
AB - The genetic stability of induced pluripotent stem (iPS) cells has a significant impact on their potential use in regenerative medicine and basic research. Analysis of the genomic integrity of iPS cells suggests a tendency to develop aberrations ranging from whole chromosome trisomies to single nucleotide mutations. Furthermore, fluctuations in telomere elongation and changes in mitochondrial DNA are also observed. Some mutations may already exist in the founder cells or result from prolonged culturing, however, many of the mutations occur during the reprogramming event. Thus, great care should be given to the initial characterization and subsequent culturing of new iPS cell lines in order to avoid the use of potentially aberrant cells.
UR - http://www.scopus.com/inward/record.url?scp=84868203369&partnerID=8YFLogxK
U2 - 10.1016/j.gde.2012.09.003
DO - 10.1016/j.gde.2012.09.003
M3 - Review article
C2 - 23040504
AN - SCOPUS:84868203369
SN - 0959-437X
VL - 22
SP - 444
EP - 449
JO - Current Opinion in Genetics and Development
JF - Current Opinion in Genetics and Development
IS - 5
ER -