TY - JOUR
T1 - Global gene expression and pigment analysis of two contrasting flower color cultivars of Canna
AU - Tripathi, Abhinandan Mani
AU - Niranjan, Abhishek
AU - Roy, Sribash
N1 - Publisher Copyright:
© 2018 Elsevier Masson SAS
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Development of flower color in plants is a complex process. Among others, it is an important trait for ornamental flowering plants. Canna is a flowering ornamental plant of family Cannaceae. To understand the molecular mechanism of flower color development in Canna, RNA sequencing from flower tissues of two contrasting flower color cultivars, Red President (RP) and Tropical Sunrise (TS) was performed. More than 27.0 million and 19.0 million clean reads were obtained from RP and TS, respectively. The combined clean reads were assembled into 147,295 unigenes. The Canna unigenes showed maximum homology with Populus trichocarpa (26.79%). A total of 2702 unigenes expressed differentially between the two cultivars of which 1972 were up-regulated and 730 were down-regulated in RP. Phenylpropanoid and flavonoid biosynthetic processes were the significant processes in RP. Expression of a vast number of transcription factors including MYB, bHLH, ARF, and WRKY were higher in RP than TS. The expression analysis of RNA sequencing data was validated by qRT-PCR analysis. Further, concentration of measured anthocyanidins and flavonols were very low or absent in TS, corroborating largely with our transcriptome data. These findings may help in understanding flower color development in Canna and in future crop breeding program.
AB - Development of flower color in plants is a complex process. Among others, it is an important trait for ornamental flowering plants. Canna is a flowering ornamental plant of family Cannaceae. To understand the molecular mechanism of flower color development in Canna, RNA sequencing from flower tissues of two contrasting flower color cultivars, Red President (RP) and Tropical Sunrise (TS) was performed. More than 27.0 million and 19.0 million clean reads were obtained from RP and TS, respectively. The combined clean reads were assembled into 147,295 unigenes. The Canna unigenes showed maximum homology with Populus trichocarpa (26.79%). A total of 2702 unigenes expressed differentially between the two cultivars of which 1972 were up-regulated and 730 were down-regulated in RP. Phenylpropanoid and flavonoid biosynthetic processes were the significant processes in RP. Expression of a vast number of transcription factors including MYB, bHLH, ARF, and WRKY were higher in RP than TS. The expression analysis of RNA sequencing data was validated by qRT-PCR analysis. Further, concentration of measured anthocyanidins and flavonols were very low or absent in TS, corroborating largely with our transcriptome data. These findings may help in understanding flower color development in Canna and in future crop breeding program.
KW - Anthocyanins
KW - Canna
KW - De-novo assembly
KW - Flavonoids
KW - Pigment
KW - Transcription factors
KW - Unigenes
UR - http://www.scopus.com/inward/record.url?scp=85043453920&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2018.03.008
DO - 10.1016/j.plaphy.2018.03.008
M3 - Article
C2 - 29544208
AN - SCOPUS:85043453920
SN - 0981-9428
VL - 127
SP - 1
EP - 10
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -