Glomerular basement membrane polyanionic sites and nitric oxide in genetically salt-sensitive and resistant hypertensive rats

Shlomit Hertzan-Levy, Adrian Iaina, Yoram Wollman, Tamara Chernihovsky, Chana Yagil, Yoram Yagil, Gary Peer, Ehud Skutelsky

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Cationic colloid gold, a polycationic histochemical probe, was used to analyze the distribution of glomerular basement membrane (GBM) polyanions, including heparan sulfate protoglycan in genetic salt-sensitive (SBH/Y) and resistant (SBN/Y) hypertensive rats, with or without high dietary salt intake. GBM morphology, renal function and nitric oxide, as measured by plasma and urine nitrite (NO2) and nitrate (NO3) were also determined. In the salt-sensitive rats the high-salt dietary intake resulted in severe hypertension, proteinuria and decreased glomerular filtration rate. After 1 month of high-salt intake, the average width of the GBM of salt-sensitive rats was higher by 27% than that of salt-resistant rats. The number of GBM anionic sites (lamina rata externa and interna) was much lower in both salt-sensitive and salt-resistant groups after 1 month of salt loading, 8.04 ± 0.36 and 7.8 ± 0.25 counts/cm, respectively, compared to the respective values of non-salt-loaded animals, 20.58 ± 1.08 counts/cm in the SBH/Y (p < 0.001) and 21 ± 1.86 counts/cm in the SBN/Y (p < 0.001). A decreased nitric oxide production was found in the salt-sensitive rats before and after salt loading compared with the salt-resistant group. No correlation was found between the nitric oxide changes and the GBM modifications. It is concluded that high-salt intake may be deleterious to the permselectivity of the GBM. It is suggested that salt restriction in hypertension may have a beneficial effect in preventing GBM permselectivity changes and proteinuria.

Original languageEnglish
Pages (from-to)218-224
Number of pages7
JournalKidney and Blood Pressure Research
Issue number4
StatePublished - 1 Jan 1997
Externally publishedYes


  • Glomerular basement membrane
  • Heparan sulfate proteoglycans
  • Hypertension
  • Polyanionic sites
  • Salt-resistant
  • Salt-sensitive

ASJC Scopus subject areas

  • Nephrology
  • Cardiology and Cardiovascular Medicine


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