Glucose determination using a re-usable enzyme-modified ion track membrane sensor

Dietmar Fink, Irina Klinkovich, Ohad Bukelman, Robert S. Marks, Arik Kiv, David Fuks, Wolfgang R. Fahrner, Lital Alfonta

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

A novel concept for a glucose biosensor based on the enzyme glucose oxidase covalently linked to nanopores of etched nuclear track membranes is presented. This device can be used to detect physiologically relevant glucose concentrations between 10 μM and 1 M. The sensitive catalytic sensor can be made re-usable due to the production of diffusible products from the oxidative biomolecular recognition event. We have demonstrated both the simplicity with which this sensor can be produced and the stability of the enzyme embedded in the nanopores. We strongly believe that the approach could be expanded for different enzymes and variable configurations of sensing and catalysis using membranes with nanopores for biocatalysis and enrichment of substrates and products. This is the first time that such devices are being used for biocatalytic transformations.

Original languageEnglish
Pages (from-to)2702-2706
Number of pages5
JournalBiosensors and Bioelectronics
Volume24
Issue number8
DOIs
StatePublished - 15 Apr 2009

Keywords

  • Etched tracks
  • Glucose oxidase
  • Glucose sensor
  • Ion track membranes
  • Nanopores

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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