Glucose‐sensitive membranes containing glucose oxidase: Activity, swelling, and permeability studies

Joseph Kost, Thomas A. Horbett, Buddy D. Ratner, Manindar Singh

Research output: Contribution to journalArticlepeer-review

206 Scopus citations

Abstract

The development of membranes that swell in response to glucose is reported. The membranes may prove to be useful in glucose monitoring or glucose‐dependent insulin delivery. The polymers were synthesized by the radiation‐induced polymerization of frozen solutions containing hydroxyethyl methacrylate, N,N‐dimethylaminoethyl methacrylate, tetraethylene glycol dimethacrylate, ethylene glycol, water, and glucose oxidase. The polymers were hydrogels, with water contents in the range of 60–90%, depending on the pH or glucose concentration. Changes in swelling and permeability of the hydrogel were caused by exposure to glucose solutions. The gluconic acid formed by the glucose oxidase catalyzed oxidation of glucose in the membrane lowered the pH of the system and thus caused the changes in the membrane. The retention of enzyme activity by the membranes in vitro and in vivo is also reported. The large differences in properties among membranes made with different chemical formulations suggest that glucose‐sensitive membranes with performance characteristics needed for an artificial pancreas may be an achievable goal.

Original languageEnglish
Pages (from-to)1117-1133
Number of pages17
JournalJournal of Biomedical Materials Research
Volume19
Issue number9
DOIs
StatePublished - 1 Jan 1985
Externally publishedYes

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