TY - JOUR
T1 - Gold nanoparticles based sensor for in vitro analysis of drug-drug interactions using imipramine and isoniazid drugs
T2 - A proof of concept approach
AU - Shaikh, Tayyaba
AU - Pandey, Ashish
AU - Sirajuddin,
AU - Talpur, Farah N.
AU - Kaushik, Aman
AU - Niazi, Javed H.
N1 - Funding Information:
This work was supported by the Scientific and Technological Research Council of Turkey (TÜBITAK), Department of Science Fellowships and Grant Programs (BIDEB) 2216 for International Researchers.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Prediction of drug-drug interactions (DDIs) before their manifestation is of paramount importance in the development of drug-dosing protocols and drug-design. In this context, we proposed for the first time, gold nanopaticles (AuNPs) based sensor for in vitro assessment of DDIs. Here, we first conducted in-situ molecular docking and simulations studies to interrogate DDIs that allowed predictions of DDIs between two model drugs, such as imipramine (antidepressant) and isoniazid (anti-tuberculosis). This was followed by in vitro studies involving imipramine conjugated gold nanoparticles (Imipramine-AuNPs) as probes for screening DDIs. Association of isoniazid with imipramine molecules over the surface of AuNPs sensor occurred that was confirmed by a combination of physico-chemical methods. Electrochemical investigations revealed the quantitative association of imipramine and isoniazid molecules, where a linear relationship of peak current (Ip) was observed for increasing concentrations of isoniazid from 31 to 667 μM at Imipramine-AuNPs modified Screen Printed Electrodes (SPE), validating the predicted DDIs. The current study aims to highlight the possible interaction between the two drugs that provides valuable information useful for the prediction of similar types of DDIs with other drug pairs.
AB - Prediction of drug-drug interactions (DDIs) before their manifestation is of paramount importance in the development of drug-dosing protocols and drug-design. In this context, we proposed for the first time, gold nanopaticles (AuNPs) based sensor for in vitro assessment of DDIs. Here, we first conducted in-situ molecular docking and simulations studies to interrogate DDIs that allowed predictions of DDIs between two model drugs, such as imipramine (antidepressant) and isoniazid (anti-tuberculosis). This was followed by in vitro studies involving imipramine conjugated gold nanoparticles (Imipramine-AuNPs) as probes for screening DDIs. Association of isoniazid with imipramine molecules over the surface of AuNPs sensor occurred that was confirmed by a combination of physico-chemical methods. Electrochemical investigations revealed the quantitative association of imipramine and isoniazid molecules, where a linear relationship of peak current (Ip) was observed for increasing concentrations of isoniazid from 31 to 667 μM at Imipramine-AuNPs modified Screen Printed Electrodes (SPE), validating the predicted DDIs. The current study aims to highlight the possible interaction between the two drugs that provides valuable information useful for the prediction of similar types of DDIs with other drug pairs.
KW - Drug-drug interaction
KW - Gold nanoparticles
KW - Imipramine
KW - Isoniazid
KW - Molecular docking
UR - http://www.scopus.com/inward/record.url?scp=85021171093&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2017.06.083
DO - 10.1016/j.snb.2017.06.083
M3 - Article
AN - SCOPUS:85021171093
SN - 0925-4005
VL - 252
SP - 1055
EP - 1062
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -