TY - JOUR
T1 - Grape berry position affects the diurnal dynamics of its metabolic profile
AU - Reshef, Noam
AU - Fait, Aaron
AU - Agam, Nurit
N1 - Funding Information:
This work was partially funded by the Koshland Foundation for the Support of Interdisciplinary Research in Combating Desertification and the Frances and Elias Margolin Trust.
Publisher Copyright:
© 2019 John Wiley & Sons Ltd
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Solar irradiance and air temperature are characterized by dramatic circadian fluctuations and are known to significantly modulate fruit composition. To date, it remains unclear whether the abrupt, yet predictive, diurnal changes in radiation and temperature prompt direct metabolic turn-over in the fruit. We assessed the role of fruit insolation, air temperature, and source-tissue CO2 assimilation in the diurnal compositional changes in ripening grape berries. This was performed by comparing the diurnal changes in metabolite profiles of berries positioned such that they experienced (a) contrasting diurnal solar irradiance patterns, and (b) similar irradiance but contrasting diurnal CO2 assimilation patterns of adjacent leaves. Grape carbon levels increased during the morning and decreased thereafter. Sucrose levels decreased throughout the day and were correlated with air temperature, but not with the diurnal pattern of leaf CO2 assimilation. Tight correlation between sucrose and glucose-6-phosphate indicated the involvement of photorespiration/glycolysis in sucrose depletion. Amino acids, polyamines, and phenylpropanoids fluctuated diurnally, and were highly responsive to the diurnal insolation pattern of the fruit. Our results fill the knowledge gap regarding the circadian pattern of source-sink assimilate-translocation in grapevine. In addition, they suggest that short-term direct solar exposure of the fruit impacts both its diurnal and nocturnal metabolism.
AB - Solar irradiance and air temperature are characterized by dramatic circadian fluctuations and are known to significantly modulate fruit composition. To date, it remains unclear whether the abrupt, yet predictive, diurnal changes in radiation and temperature prompt direct metabolic turn-over in the fruit. We assessed the role of fruit insolation, air temperature, and source-tissue CO2 assimilation in the diurnal compositional changes in ripening grape berries. This was performed by comparing the diurnal changes in metabolite profiles of berries positioned such that they experienced (a) contrasting diurnal solar irradiance patterns, and (b) similar irradiance but contrasting diurnal CO2 assimilation patterns of adjacent leaves. Grape carbon levels increased during the morning and decreased thereafter. Sucrose levels decreased throughout the day and were correlated with air temperature, but not with the diurnal pattern of leaf CO2 assimilation. Tight correlation between sucrose and glucose-6-phosphate indicated the involvement of photorespiration/glycolysis in sucrose depletion. Amino acids, polyamines, and phenylpropanoids fluctuated diurnally, and were highly responsive to the diurnal insolation pattern of the fruit. Our results fill the knowledge gap regarding the circadian pattern of source-sink assimilate-translocation in grapevine. In addition, they suggest that short-term direct solar exposure of the fruit impacts both its diurnal and nocturnal metabolism.
KW - circadian cycle
KW - diurnal metabolism
KW - fruit metabolic profiling
KW - fruit microclimate
KW - fruit sunlight exposure
KW - source/sink translocation
UR - http://www.scopus.com/inward/record.url?scp=85062801691&partnerID=8YFLogxK
U2 - 10.1111/pce.13522
DO - 10.1111/pce.13522
M3 - Article
C2 - 30673142
AN - SCOPUS:85062801691
SN - 0140-7791
VL - 42
SP - 1897
EP - 1912
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 6
ER -