Green synthesis of gold nanoparticles using plant extracts as reducing agents

Paz Elia, Raya Zach, Sharon Hazan, Sofiya Kolusheva, Ze'ev Porat, Yehyda Zeiri

Research output: Contribution to journalArticlepeer-review

316 Scopus citations


Gold nanoparticles (GNPs) were prepared using four different plant extracts as reducing and stabilizing agents. The extracts were obtained from the following plants: Salvia officinalis, Lippia citriodora, Pelargonium graveolens and Punica granatum. The size distributions of the GNPs were measured using three different methods: dynamic light scattering, nanoparticle-tracking analysis and analysis of scanning electron microscopy images. The three methods yielded similar size distributions. Biocompatibility was examined by correlation of L-cell growth in the presence of different amounts of GNPs. All GNPs showed good biocompatibility and good stability for over 3 weeks. Therefore, they can be used for imaging and drug-delivery applications in the human body. High-resolution transmission electron microscopy was used to view the shapes of the larger GNPs, while infrared spectroscopy was employed to characterize the various functional groups in the organic layer that stabilize the particles. Finally, active ingredients in the plant extract that might be involved in the formation of GNPs are proposed, based on experiments with pure antioxidants that are known to exist in that plant.

Original languageEnglish
Pages (from-to)4007-4021
Number of pages15
JournalInternational Journal of Nanomedicine
Issue number1
StatePublished - 20 Aug 2014


  • Antioxidants
  • Gold nanoparticles
  • Lippia citriodora
  • Pelargonium graveolens
  • Punica granatum
  • Salvia officinalis
  • Size distribution
  • Zeta potential

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry


Dive into the research topics of 'Green synthesis of gold nanoparticles using plant extracts as reducing agents'. Together they form a unique fingerprint.

Cite this