Haloferax volcanii AglB and AglD are Involved in N-glycosylation of the S-layer Glycoprotein and Proper Assembly of the Surface Layer

Mehtap Abu-Qarn, Sophie Yurist-Doutsch, Assunta Giordano, Andrej Trauner, Howard R. Morris, Paul Hitchen, Ohad Medalia, Anne Dell, Jerry Eichler

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

In this study, the effects of deleting two genes previously implicated in Haloferax volcanii N-glycosylation on the assembly and attachment of a novel Asn-linked pentasaccharide decorating the H. volcanii S-layer glycoprotein were considered. Mass spectrometry revealed the pentasaccharide to comprise two hexoses, two hexuronic acids and an additional 190 Da saccharide. The absence of AglD prevented addition of the final hexose to the pentasaccharide, while cells lacking AglB were unable to N-glycosylate the S-layer glycoprotein. In AglD-lacking cells, the S-layer glycoprotein-based surface layer presented both an architecture and protease susceptibility different from the background strain. By contrast, the absence of AglB resulted in enhanced release of the S-layer glycoprotein. H. volcanii cells lacking these N-glycosylation genes, moreover, grew significantly less well at elevated salt levels than did cells of the background strain. Thus, these results offer experimental evidence showing that N-glycosylation endows H. volcanii with an ability to maintain an intact and stable cell envelope in hypersaline surroundings, ensuring survival in this extreme environment.

Original languageEnglish
Pages (from-to)1224-1236
Number of pages13
JournalJournal of Molecular Biology
Volume374
Issue number5
DOIs
StatePublished - 14 Dec 2007

Keywords

  • Archaea
  • N-glycosylation
  • glycosyltransferase
  • oligosaccharyltransferase
  • surface-layer glycoprotein

Fingerprint

Dive into the research topics of 'Haloferax volcanii AglB and AglD are Involved in N-glycosylation of the S-layer Glycoprotein and Proper Assembly of the Surface Layer'. Together they form a unique fingerprint.

Cite this