Heat‐shock protein 83 of Leishmania mexicana amazonensis is an abundant cytoplasmic protein with a tandemly repeated genomic arrangement

Michal SHAPIRA, Elena PINELLI

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2 Scopus citations

Abstract

The 83‐kDa heat‐shock protein HSP83 is a highly abundant protein in Leishmania amastigotes and promastigotes exposed to elevated temperature. Antibodies against this protein were obtained by immunization with a synthetic peptide derived from a conserved region. These antibodies both the denatured and the native form of the molecule and were used for immunofluorescence analysis. These experiments, together with analysis by cell fractionation, show that HSP83 is distributed in the cytoplasm of Leishmania parasites. The gene for HSP83 in Leishmania mexicana amazonensis has been cloned from a genomic library, and molecular characterization shows it is present in several copies of 4‐kb repeats arranged in tandem.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalEuropean Journal of Biochemistry
Volume185
Issue number2
DOIs
StatePublished - 1 Jan 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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