The glucose transport properties of brush border membrane vesicles from the outer cortex (early proximal tubule) and outer medulla (late proximal tubule) of rabbit kidney were studied. In the outer cortical preparation the behavior of the sodium-dependent component of D-glucose flux indicated the presence of a low-affinity transport system with K(m) ≃ 6 mM and V(max) ≃ 10 nmol . min-1 . mg protein-1 as measured under zero trans conditions at 40 mM NaC1 and 17°C. By contrast, in the outer medullary preparation this component of flux behaved as a high-affinity system with K(m) ≃ 0.35 mM and V(max) ≃ 4 nmol . min-1 . mg protein-1. Differences in transport specificity between the two preparations were also indicated and glucose uptake by the outer cortical vesicles was significantly more sensitive to inhibition by phlorizin. These results suggest the existence of two distinct sodium-dependent D-glucose transport systems in the renal proximal tubule brush border membrane. The kinetic studies presented here were done under zero trans sodium and glucose conditions. The rationale and methodology for carrying out these measurements reliably are discussed in detail.
|Journal||American Journal of Physiology - Renal Fluid and Electrolyte Physiology|
|State||Published - 1 Jan 1982|
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