High-affinity Ca2+-binding site inhibiting Ca2+ release from sarcoplasmic reticulum

Anat Argaman, Varda Shoshan-Barmatz

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Ca2+ release from sarcoplasmic reticulum membranes, activated by alkaline pH occurs only when EGTA is present in the release medium. Addition of very low concentrations of Ca2+ to the medium inhibits Ca2+ release. The concentration of free Ca2+ required for 50% inhibition ranges from between 5 and 20 nM in different experiments and/or membrane preparations, irrespective of whether the free Ca2+ concentration is controlled by EGTA or CDTA. Other divalent cations such as Mn2+, Ba2+, Cu2+, Cd2+ and Mg2+ also exert an inhibitory effect on Ca2+ release, with higher or lower potency than that of Ca2+. The inactivation of Ca2+ release by Ca2+ is reversible. We suggest the involvement of high-affinity Ca2+-binding sites in the control of Ca2+ release.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalFEBS Letters
Issue number1
StatePublished - 16 Jan 1989


  • Ca release
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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